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Updated: Jun 10, 2026

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions
Published on: August 2, 2018
[Hypoxia enhances the proliferation of skeletal myoblasts and possible mechanism]
Fan Wang1, Ling-ling Zhu, Xiao-ping Chen
1Department of Geriatric Cardiology, the PLA General Hospital, Beijing 100854, China. fanming@nic.bmi.ac.cn
Objective:
To explore the role of hypoxia in skeletal myoblasts proliferation and related mechanism.
Methods:
The numbers and proliferous indexes of skeletal myoblasts were detected by flow cytometer under 20%, 3%, and 10% oxygen concentration. Hypoxia inducing factor 1alpha (HIF-1alpha) mRNA expression was determined by reverse transcriptase-polymerase chain reaction (RT-PCR). HIF-1alpha proteinum in endochylema and intranucelus were respectively detected by Western blot.
Results:
The numbers and proliferous indexes were higher (P < 0.05) in hypoxia group than those of control group. The expression HIF-1alpha mRNA had no difference in hypoxia and in normal groups. The level of HIF-1alpha proteinum in endochylema under normoxia was more than that in intranucelus and it was opposite under hypoxia.
Conclusion:
Hypoxia can promote the proliferation of skeletal myoblasts. The possible mechanism of hypoxia promoting the proliferation of skeletal myoblasts might be that low oxygen concentration regulates HIF-la nuclear translocation.
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