Related Experiment Video
Updated: Jun 10, 2026

Standardized Identification of Compound Structure in Tibetan Medicine Using Ion Trap Mass Spectrometry and Multiple-Stage Fragmentation Analysis
Published on: March 17, 2023
Characterization of emtricitabine related substances by liquid chromatography coupled to an ion trap mass
Murali Pendela1, Dunge Ashenafi Mamade, Jos Hoogmartens
1Katholieke Universiteit Leuven, Faculteit Farmaceutische Wetenschappen, Laboratorium voor Farmaceutische Analyse, O&N2, PB 923, Herestraat 49, B-3000 Leuven, Belgium.
Abstract:
Emtricitabine (FTC) is an antiretroviral compound that inhibits the HIV-1 reverse transcriptase. For the quantification of FTC related substances, a liquid chromatography (LC) method coupled with ultraviolet (UV) detection was developed earlier in our laboratory. Several unknown compounds were detected during the analysis of a commercial sample. However, most of these impurities were not characterized. In this study, impurity profiling in a selected FTC sample was done using LC-mass spectrometry (MS). Due to the presence of a non-volatile buffer, a desalting procedure was carried out before sending the impurity into the MS. Totally, nine peaks were studied and most of them could be characterized.
Related Concept Videos
Electrospray Ionization (ESI) Mass Spectrometry
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
Chemical Ionization (CI) Mass Spectrometry
Ion-Exchange Chromatography
Tandem Mass Spectrometry
High-Performance Liquid Chromatography: Types of Detectors
Peptide Identification Using Tandem Mass Spectrometry
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
