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Updated: Jun 10, 2026

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
Published on: December 25, 2015
[Isolation, identification and characterization of a high-efficient heterotrophic nitrifier]
Qingmei Zeng1, Wengong Si, Zhiqiang Li
1Engineering Research Center of Bio-process, Ministry of Education, Hefei University of Technology, Hefei 230009, China. zengqingmei-1@163.com
A novel heterotrophic nitrifier, Alcaligenes sp. HN-S, efficiently removes ammonia nitrogen from wastewater. This bacterium demonstrates a high removal rate and optimal conditions for nitrogen removal, supporting new biological treatment processes.
Area of Science:
- Environmental Microbiology
- Biotechnology
- Wastewater Treatment
Context:
- Heterotrophic nitrification is crucial for nitrogen removal in wastewater treatment.
- Existing methods often face limitations in efficiency and rate.
- Screening for high-performance microorganisms is essential for process optimization.
Purpose:
- To isolate and identify a highly efficient heterotrophic nitrifier.
- To characterize its nitrification capabilities and optimal growth conditions.
- To evaluate its potential for practical wastewater treatment applications.
Summary:
- A gram-negative bacillus, identified as Alcaligenes sp. HN-S, was isolated from activated sludge.
- This strain exhibited a high ammonia nitrogen removal efficiency (99.8%) and a rapid removal rate (9.61 mg-N/L/h).
- Optimal conditions for nitrification include sodium citrate as a carbon source, suitable dissolved oxygen levels, a favorable C/N ratio, temperatures between 30-35°C, and pH 5.0-9.0.
Impact:
- Alcaligenes sp. HN-S offers a significant advancement in ammonia nitrogen removal rates compared to previously identified nitrifiers.
- The study provides critical theoretical support for developing novel, high-efficiency biological nitrogen removal processes.
- This research contributes to more sustainable and effective wastewater management strategies.
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