The StrataTest® human skin model, a consistent in vitro alternative for toxicological testing

Area of Science:

• In vitro toxicology
• Dermatology
• Biotechnology

Background:

• Three-dimensional in vitro skin models offer an alternative to animal testing for assessing chemical and physical damage.
• The StrataTest® human skin model, utilizing NIKS® keratinocyte progenitors, is a fully-stratified tissue with epidermal and dermal components.
• This model exhibits barrier function, validated by electrical impedance measurements.

Purpose of the Study:

• To evaluate the utility of the StrataTest® human skin model for toxicological assessments.
• To determine the model's consistency and applicability in detecting skin damage and the efficacy of protective agents.

Main Methods:

• The StrataTest® model's barrier function was assessed using electrical impedance.
• Skin tissues were exposed to ozone, cigarette smoke, and ultraviolet (UV) irradiation.
• Reactive oxygen species (ROS), Interleukin (IL)-1α, IL-1 receptor antagonist (IL-1RA), and thymidine dimer formation were measured.
• The effects of parthenolide-depleted (PD)-Feverfew extract and a UVA/UVB sunscreen were investigated.

Main Results:

• The StrataTest® model demonstrated consistent responses to chemical irritants after refrigerated storage.
• Exposure to ozone, cigarette smoke, and UV irradiation induced ROS production.
• UV irradiation increased IL-1α and IL-1RA secretion and thymidine dimer formation.
• Pretreatment with PD-Feverfew extract or sunscreen mitigated these UV-induced effects.

Conclusions:

• The StrataTest® human skin model is a robust and reproducible tool for in vitro toxicological evaluations.
• The model effectively assesses skin damage from various agents and the protective capabilities of mitigating compounds.
• Its broad applicability supports its use in a wide range of toxicological assays, reducing reliance on animal testing.