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Updated: Jun 10, 2026
A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.
Published on: November 7, 2018
DNA microarray for tracing Salmonella in the feed chain
Sevinc Koyuncu1, Gunnar Andersson, Pieter Vos
1Department of Chemistry, Environment and Feed Hygiene, National Veterinary Institute, SE-751 89 Uppsala, Sweden. sevinc.koyuncu@sva.se
Abstract:
In the present study we investigated if the microarray platforms Premi®Test Salmonella (PTS) and Salmonella array (SA) could be applied for the identification and typing of Salmonella in artificially contaminated animal feed materials. The results were compared to the culture-based MSRV method and serotyping according to Kauffman-White. The SA platform showed a specificity of 100% for the identification of Salmonella compared to 93% with the PTS platform and a sensitivity of 99% or 100%, respectively. Among all identified Salmonella serotypes, 56% with the SA platform and 81% with the PTS platform were correctly identified. The difference in probe signal intensity for each probe was higher between duplicates analyzed with the SA platform than with the PTS platform. Attempts to use the microarray platforms from BPW resulted in many false negative samples and incorrect typing results. The microarray platforms tested were simple to use and might have a potential in tracing studies for Salmonella in the feed chain particularly when rapid information about serotypes are important.
Insights
The Salmonella array (SA) platform demonstrated superior specificity and sensitivity for identifying Salmonella in animal feed compared to the Premi®Test Salmonella (PTS) platform. Both platforms showed potential for Salmonella tracing in feed chains, with SA offering better Salmonella serotype identification.
Area of Science:
- Food Microbiology
- Molecular Diagnostics
- Veterinary Science
Background:
- Salmonella contamination in animal feed poses a significant risk to animal and human health.
- Accurate and rapid detection methods are crucial for controlling Salmonella spread in the feed chain.
- Traditional culture-based methods can be time-consuming, necessitating faster diagnostic tools.
Purpose of the Study:
- To evaluate the performance of two microarray platforms, Premi®Test Salmonella (PTS) and Salmonella array (SA), for Salmonella identification and typing in artificially contaminated animal feed.
- To compare the accuracy of these platforms against established methods like MSRV and Kauffman-White serotyping.
- To assess the suitability of microarray technology for Salmonella surveillance in animal feed.
Main Methods:
- Artificially contaminated animal feed samples were analyzed using PTS and SA microarray platforms.
- Results were validated against the culture-based MSRV method and Kauffman-White serotyping.
- Specificity, sensitivity, and accuracy of Salmonella serotype identification were calculated for each platform.
- Probe signal intensity differences were analyzed to assess platform reproducibility.
Main Results:
- The SA platform exhibited 100% specificity and 99% sensitivity for Salmonella identification, outperforming the PTS platform (93% specificity, 100% sensitivity).
- SA correctly identified 56% of Salmonella serotypes, while PTS identified 81% correctly.
- Microarray analysis directly from Buffered Peptone Water (BPW) enrichment resulted in numerous false negatives and inaccurate typing.
- SA showed greater consistency between duplicate analyses compared to PTS.
Conclusions:
- The Salmonella array (SA) platform is a highly specific and sensitive tool for Salmonella detection in animal feed.
- While PTS showed higher serotype identification accuracy in this study, SA's overall performance suggests its utility.
- Direct analysis from BPW is not recommended due to unreliable results.
- Microarray platforms, particularly SA, show promise for rapid Salmonella tracing in the animal feed supply chain.
