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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

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A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.
06:55

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Published on: November 7, 2018

DNA microarray for tracing Salmonella in the feed chain.

Sevinc Koyuncu1, Gunnar Andersson, Pieter Vos

  • 1Department of Chemistry, Environment and Feed Hygiene, National Veterinary Institute, SE-751 89 Uppsala, Sweden. sevinc.koyuncu@sva.se

International Journal of Food Microbiology
|August 7, 2010
PubMed
Summary

The Salmonella array (SA) platform demonstrated superior specificity and sensitivity for identifying Salmonella in animal feed compared to the Premi®Test Salmonella (PTS) platform. Both platforms showed potential for Salmonella tracing in feed chains, with SA offering better Salmonella serotype identification.

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Area of Science:

  • Food Microbiology
  • Molecular Diagnostics
  • Veterinary Science

Background:

  • Salmonella contamination in animal feed poses a significant risk to animal and human health.
  • Accurate and rapid detection methods are crucial for controlling Salmonella spread in the feed chain.
  • Traditional culture-based methods can be time-consuming, necessitating faster diagnostic tools.

Purpose of the Study:

  • To evaluate the performance of two microarray platforms, Premi®Test Salmonella (PTS) and Salmonella array (SA), for Salmonella identification and typing in artificially contaminated animal feed.
  • To compare the accuracy of these platforms against established methods like MSRV and Kauffman-White serotyping.
  • To assess the suitability of microarray technology for Salmonella surveillance in animal feed.

Main Methods:

  • Artificially contaminated animal feed samples were analyzed using PTS and SA microarray platforms.
  • Results were validated against the culture-based MSRV method and Kauffman-White serotyping.
  • Specificity, sensitivity, and accuracy of Salmonella serotype identification were calculated for each platform.
  • Probe signal intensity differences were analyzed to assess platform reproducibility.

Main Results:

  • The SA platform exhibited 100% specificity and 99% sensitivity for Salmonella identification, outperforming the PTS platform (93% specificity, 100% sensitivity).
  • SA correctly identified 56% of Salmonella serotypes, while PTS identified 81% correctly.
  • Microarray analysis directly from Buffered Peptone Water (BPW) enrichment resulted in numerous false negatives and inaccurate typing.
  • SA showed greater consistency between duplicate analyses compared to PTS.

Conclusions:

  • The Salmonella array (SA) platform is a highly specific and sensitive tool for Salmonella detection in animal feed.
  • While PTS showed higher serotype identification accuracy in this study, SA's overall performance suggests its utility.
  • Direct analysis from BPW is not recommended due to unreliable results.
  • Microarray platforms, particularly SA, show promise for rapid Salmonella tracing in the animal feed supply chain.