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Updated: Jun 10, 2026

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer
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Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer

Published on: June 10, 2022

Protocol for constructing tissue arrays by cutting edge matrix assembly.

Thai Hong Tran1, Justin Lin, Ashley Brooke Sjolund

  • 1Department of Cancer Biology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|August 7, 2010
PubMed
Summary
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We developed a novel protocol for high-density tissue microarrays using scaffold-free matrix assembly. This cutting-edge technique maximizes array density, enabling over 10,000 tissue features per slide for advanced analysis.

Area of Science:

  • Biotechnology
  • Histology
  • Pathology

Background:

  • Tissue microarrays (TMAs) are crucial for high-throughput analysis.
  • Existing TMA construction methods can be limited by space efficiency and density.

Purpose of the Study:

  • To present a novel protocol for constructing high-density tissue microarrays.
  • To maximize the number of tissue samples on a single slide.

Main Methods:

  • A scaffold-free, self-supporting matrix assembly technique.
  • Repetitive sectioning and edge-to-edge bonding of rectangular tissue plates.
  • Layered construction creating a 'laminate of laminates' array block.

Main Results:

  • Achieved maximized array density with minimal wasted space.

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Directed Cellular Self-Assembly to Fabricate Cell-Derived Tissue Rings for Biomechanical Analysis and Tissue Engineering
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Published on: November 25, 2011

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Last Updated: Jun 10, 2026

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer
12:03

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer

Published on: June 10, 2022

Directed Cellular Self-Assembly to Fabricate Cell-Derived Tissue Rings for Biomechanical Analysis and Tissue Engineering
08:00

Directed Cellular Self-Assembly to Fabricate Cell-Derived Tissue Rings for Biomechanical Analysis and Tissue Engineering

Published on: November 25, 2011

  • Successfully constructed tissue array blocks with over 10,000 tissue features.
  • Enabled micrometer-thin sectioning for detailed array display and analysis.

    • Conclusions:

    • The presented protocol enables unprecedented high-density tissue microarray construction.
    • This technology significantly enhances the capacity for large-scale tissue-based studies.
    • Facilitates advanced research in histology, pathology, and biomarker discovery.