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Related Experiment Video

Updated: Jun 10, 2026

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer
12:03

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer

Published on: June 10, 2022

An alternative technology to prepare tissue microarray using frozen tissue samples.

Zhongting Hu1, Elbert Chang, Melissa Hodeib

  • 1Department of Anatomy, College of Osteopathic Medicine of the Pacific, Western University of Health Sciences, Pomona, CA, USA. zhongting@yahoo.com

Methods in Molecular Biology (Clifton, N.J.)
|August 7, 2010
PubMed
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This study presents a new, low-cost method for creating tissue microarrays (TMAs) from frozen tissues. This technique preserves high-quality RNA and proteins, improving gene expression analysis for research.

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Histology

Background:

  • Tissue microarrays (TMAs) are crucial for high-throughput molecular analyses.
  • Current TMA methods primarily use paraffin-embedded tissues, which can degrade RNA and protein quality.
  • Frozen tissues offer superior RNA and protein integrity for gene expression profiling, but TMA preparation from them is less common.

Purpose of the Study:

  • To introduce a novel, accessible method for preparing TMAs from a wider range of frozen tissue samples.
  • To provide an alternative to existing TMA techniques that better preserves molecular integrity.

Main Methods:

  • Development of an agarose-gel-based recipient block using simple instruments.
  • Manual coring and arraying of frozen donor tissues into the recipient block at -10°C.

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Working with Human Tissues for Translational Cancer Research
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Related Experiment Videos

Last Updated: Jun 10, 2026

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer
12:03

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer

Published on: June 10, 2022

Working with Human Tissues for Translational Cancer Research
07:48

Working with Human Tissues for Translational Cancer Research

Published on: November 26, 2015

  • Direct sectioning of the array block on a cryostat microtome for slide preparation.

    • Main Results:

    • The developed method yields TMAs with well-defined array configurations.
    • Tissue and cell morphology are well-preserved in the resulting TMA slides.
    • Proteins and messenger RNAs (mRNAs) are well-preserved, suitable for molecular analyses.

    Conclusions:

    • This agarose-gel-based method offers a low-cost, time-saving alternative for high-quality TMA preparation from frozen tissues.
    • The method is suitable for in situ hybridization and immunocytochemistry studies.
    • It enhances the utility of frozen tissues for gene expression analyses.