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Related Concept Videos

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...

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Related Experiment Video

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In Vivo Augmentation of Gut-Homing Regulatory T Cell Induction
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Increased CD127 expression on activated FOXP3+CD4+ regulatory T cells.

Federico Simonetta1, Amel Chiali, Corinne Cordier

  • 1INSERM, U1012, Le Kremlin-Bicêtre, France.

European Journal of Immunology
|August 7, 2010
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Low CD127 (IL-7R alpha) expression is not an intrinsic trait of regulatory T cells (Treg). Activated Treg subsets upregulate CD127, suggesting they are a target for IL-7 therapies.

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Published on: December 30, 2016

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Regulatory T cells (Treg) are crucial for immune homeostasis.
  • Treg are typically identified by CD25 and FOXP3 expression and low CD127 (IL-7R alpha).
  • Treg function is considered independent of IL-7 in the periphery.

Purpose of the Study:

  • To investigate CD127 expression dynamics on Treg.
  • To determine if Treg activation influences CD127 expression.
  • To explore the functional implications of altered CD127 expression on Treg.

Main Methods:

  • Analysis of CD127 expression on distinct Treg subsets.
  • In vitro and in vivo Treg activation studies.
  • Adoptive transfer and contact dermatitis models.
  • Assessment of STAT5 phosphorylation and cell survival upon IL-7 exposure.

Main Results:

  • Differential CD127 expression observed across Treg subsets.
  • Activated Treg significantly upregulate CD127 compared to conventional T cells.
  • High CD127 expression on Treg found ex vivo in bone marrow and skin.
  • Increased CD127 expression correlates with enhanced STAT5 signaling and survival upon IL-7 stimulation.

Conclusions:

  • Low CD127 expression is not a defining characteristic of all Treg.
  • Activated Treg exhibit high CD127 expression, indicating responsiveness to IL-7.
  • Activated Treg represent a potential target for IL-7-based therapies.