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Updated: Jun 10, 2026

Ultra-High-Speed Western Blot using Immunoreaction Enhancing Technology
Published on: September 26, 2020
An economical single-sided antibody incubation method for Western blotting
Chuanying Pan1, Xianyong Lan, Hong Chen
1College of Life Sciences, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, China.
Abstract:
A simple, single-sided antibody method for incubating primary and secondary antibodies in Western blotting was developed, which generates significant savings on the use of antibodies. Compared with the conventional immersion technique for antibody incubation, the present economical single-sided antibody incubation method resulted in a saving of 80% of antibody use. Besides, the present incubation method did not compromise the Western blot results and was not affected by the expression levels of target proteins.
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