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Updated: Jun 10, 2026

Contrast Imaging in Mouse Embryos Using High-frequency Ultrasound

Contrast Imaging in Mouse Embryos Using High-frequency Ultrasound

Published on: March 4, 2015

Imaging mouse embryonic development.

Ryan S Udan¹, Mary E Dickinson

¹Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, Texas, USA.

Methods in Enzymology

|August 10, 2010

Summary

This summary is machine-generated.

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Researchers can now use time-lapse imaging to observe early mouse embryo development in real time. This technique, utilizing fluorescent proteins, enhances understanding of embryogenesis and cell dynamics.

Area of Science:

Developmental Biology
Cell Biology
Genetics

Background:

Optimized ex vivo mouse embryo culture methods have been developed over 30 years to improve viability and physiology during embryogenesis.
Advances in transgenic animal production and fluorescent protein (FP) development have spurred the use of time-lapse imaging in developmental studies.

Purpose of the Study:

To provide a comprehensive guide for performing time-lapse imaging of early mouse embryo development.
To highlight the utility of fluorescent proteins in visualizing dynamic cellular events during embryogenesis.

Main Methods:

Utilizing optimized ex vivo embryo culture techniques.
Employing time-lapse microscopy combined with fluorescent protein labeling.
Targeting fluorescent proteins to specific cell types or subcellular compartments for detailed observation.

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Live Imaging of Early Cardiac Progenitors in the Mouse Embryo

Live Imaging of Early Cardiac Progenitors in the Mouse Embryo

Published on: July 12, 2022

Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex

Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex

Published on: June 4, 2014

Related Experiment Videos

Last Updated: Jun 10, 2026

Contrast Imaging in Mouse Embryos Using High-frequency Ultrasound

Contrast Imaging in Mouse Embryos Using High-frequency Ultrasound

Published on: March 4, 2015

Live Imaging of Early Cardiac Progenitors in the Mouse Embryo

Live Imaging of Early Cardiac Progenitors in the Mouse Embryo

Published on: July 12, 2022

Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex

Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex

Published on: June 4, 2014

Main Results:

Real-time visualization of dynamic events such as cell proliferation, apoptosis, and migration in living mouse embryos.
Detailed analysis of morphological changes in developing embryos.
Successful application of time-lapse imaging to study critical stages of mouse embryogenesis.

Conclusions:

Time-lapse imaging is a powerful tool for studying dynamic cellular processes during early mouse development.
Fluorescent protein technology significantly enhances the ability to track and analyze developmental events in real time.
This guide facilitates the application of these advanced imaging techniques in developmental biology research.