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Updated: Jun 10, 2026
X-Ray Crystallography to Study the Oligomeric State Transition of the Thermotoga maritima M42 Aminopeptidase TmPep1050
Published on: May 13, 2020
Expression, purification, crystallization and preliminary X-ray crystallographic data from TktA, a transketolase from
Matt Horsham1, Harriet Saxby, James Blake
1FBLS I&I, University of Glasgow, 120 University Place, Glasgow G12 8TA, Scotland.
Abstract:
The enzyme transketolase from the lactic acid bacterium Lactobacillus salivarius (subsp. salivarius UCC118) has been recombinantly expressed and purified using an Escherichia coli expression system. Purified transketolase from L. salivarius has been crystallized using the vapour-diffusion technique. The crystals belonged to the trigonal space group P3(2)21, with unit-cell parameters a=b=75.43, c=184.11 A, and showed diffraction to 2.3 A resolution.
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