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Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Protein-protein Interfaces02:04

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Jun 10, 2026

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
08:07

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

Small molecule protein interaction profiling with functional protein microarrays.

Lihao Meng1, Dawn Mattoon, Paul Predki

  • 1Life Technologies Corporation, Carlsbad, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|August 10, 2010
PubMed
Summary
This summary is machine-generated.

Protein microarray technology enables efficient identification of small molecule targets and assessment of drug selectivity. This method offers a scalable and sensitive approach for drug development, overcoming limitations of traditional techniques.

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Identifying Protein-protein Interaction Sites Using Peptide Arrays
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Identifying Protein-protein Interaction Sites Using Peptide Arrays

Published on: November 18, 2014

Related Experiment Videos

Last Updated: Jun 10, 2026

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
08:07

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions
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Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

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Identifying Protein-protein Interaction Sites Using Peptide Arrays
07:44

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Published on: November 18, 2014

Area of Science:

  • Biochemistry
  • Chemical Biology
  • Drug Discovery

Background:

  • Small molecules modulate protein functions for research and therapeutics.
  • Current mass spectrometry methods for target identification are limited in throughput and resource efficiency.
  • Existing technologies struggle to broadly assess small molecule selectivity and off-target effects.

Purpose of the Study:

  • To apply protein microarray technology for studying small molecule-protein interactions.
  • To develop a sensitive and scalable method for small molecule selectivity profiling.
  • To overcome limitations of current target identification and selectivity assessment techniques.

Main Methods:

  • Utilizing protein microarray technology to detect small molecule-protein interactions.
  • Assessing assay sensitivity for interactions with low micromolar affinity.
  • Evaluating assay reproducibility, sensitivity, and scalability.

Main Results:

  • Demonstrated protein microarray technology's capability to study small molecule-protein interactions.
  • Achieved sensitivity sufficient for detecting interactions with low micromolar affinity.
  • Developed highly reproducible, sensitive, and scalable assays.

Conclusions:

  • Protein microarray technology is a powerful tool for small molecule-protein interaction studies.
  • This approach enables comprehensive small molecule selectivity profiling.
  • The technology facilitates advancements in drug development by addressing key limitations in current methods.