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Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery
Published on: May 2, 2019
PEGylated iminodiacetic acid zinc complex stabilizes cationic RNA-bearing nanoparticles
Daniel E Levy1, Zhongli Ding, Chiwei Hu
1Intradigm Corporation, 3350 West Bayshore Road, Suite 100, Palo Alto, CA 94303, USA. del345@gmail.com
Abstract:
Self-assembling nanoparticles comprising cationic polymers are of interest for the delivery of oligonucleotide-based therapeutics. Unfortunately, exposure of the nanoparticle cationic surface to plasma and plasma proteins compromises particle stability and circulating half-life. Herein, we report that improved nanoparticle stability can be achieved through temporary grafting of PEG to the nanoparticle surface. Grafting is induced through zinc complexation between PEG-IDA and the exposed polyhistidylated polylysine (H-K) cationic polymer of pre-formed nanoparticles.
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