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Related Experiment Video

Updated: Jun 10, 2026

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer
12:03

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer

Published on: June 10, 2022

Tissue microarray construction from gross specimens: development of a novel simple technique.

Santosh Kumar Sharma1, Lopamudra Deka, Ruchika Gupta

  • 1Department of Pathology, Hindu Rao Hospital, New Delhi, India.

Journal of Clinical Pathology
|August 11, 2010
PubMed
Summary

This study presents an affordable method for creating tissue microarrays (TMAs) from gross specimens. This cost-effective technique enables widespread use of TMAs in pathology research and quality control, even in resource-limited settings.

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Area of Science:

  • Surgical Pathology
  • Oncology
  • Biotechnology

Background:

  • Tissue microarray (TMA) is valuable for research and quality control in surgical pathology.
  • High cost of commercial instruments limits widespread TMA adoption, especially in developing countries.
  • A novel, inexpensive method for constructing TMAs from gross specimens is presented.

Purpose of the Study:

  • To describe an economical technique for building tissue microarrays (TMAs) from gross tumor specimens.
  • To validate the utility of this novel TMA construction method for immunohistochemistry.

Main Methods:

  • A 16-gauge bone marrow trephine biopsy needle was used to obtain cores from 150 breast carcinoma specimens.
  • Cores were processed, arrayed into a recipient block, and used for immunohistochemistry.

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A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies
09:32

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies

Published on: September 23, 2014

Related Experiment Videos

Last Updated: Jun 10, 2026

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer
12:03

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer

Published on: June 10, 2022

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies
09:32

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies

Published on: September 23, 2014

  • Results were compared with conventional whole tissue sections.

    • Main Results:

    • Satisfactory preservation was achieved in 148 out of 150 TMA blocks (98.7%).
    • Immunohistochemical staining for ER, PR, Her2.neu, and Ki-67 was successful.
    • Staining intensity was comparable to conventional tissue sections.

    Conclusions:

    • The described technique provides a satisfactory method for TMA construction from gross specimens.
    • This approach is suitable for high-volume centers, particularly for breast carcinoma.
    • Further validation across other tumor types is recommended.