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Related Experiment Video

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Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
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Liquid dielectrophoresis and surface microfluidics.

Karan V I S Kaler1, Ravi Prakash, Dipankar Chugh

  • 1Department of Electrical and Computer Engineering, Schulich School of Engineering, University of Calgary, Alberta, Canada T2N-1N4.

Biomicrofluidics
|August 11, 2010
PubMed
Summary

Liquid dielectrophoresis (L-DEP) enables rapid, automated manipulation of small aqueous samples on hydrophobic surfaces for microfluidic and lab-on-a-chip applications. This technology is valuable for on-chip bioassays and multiplexed diagnostic screening.

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Area of Science:

  • Microfluidics
  • Biotechnology
  • Surface Science

Background:

  • Liquid dielectrophoresis (L-DEP) offers precise manipulation of polar aqueous samples at microscopic scales.
  • Hydrophobic surfaces facilitate L-DEP for microfluidic and lab-on-a-chip (LOC) device development.
  • Surface microfluidic (SMF) devices leverage L-DEP for advanced sample handling.

Purpose of the Study:

  • To review recent advancements and applications of L-DEP in handling biological aqueous samples.
  • To highlight the utility of L-DEP-based SMF devices for on-chip bioassays, including nucleic acid analysis.
  • To explore the potential of L-DEP for automated multiplexed assays in diagnostics and screening.

Main Methods:

  • Deployment of L-DEP at microscopic scales on hydrophobic surfaces.
  • Utilization of microchip-based surface microfluidic (SMF) devices.
  • Integration of on-chip or off-chip detection capabilities.

Main Results:

  • Demonstrated effective handling and processing of diverse aqueous samples and reagents, including emulsions.
  • Showcased the utility of L-DEP-based SMF devices for on-chip bioassays and nucleic acid analysis.
  • Highlighted parallel sample processing capabilities for automated assays.

Conclusions:

  • L-DEP on hydrophobic surfaces provides a powerful platform for microfluidic applications and LOC devices.
  • L-DEP-based SMF devices are suitable for sensitive on-chip bioassays and nucleic acid analysis.
  • The parallel processing capability of L-DEP-based SMF devices supports high-throughput automated multiplexed diagnostic and screening assays.