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Updated: Jun 10, 2026
Generation of Human Monocyte-derived Dendritic Cells from Whole Blood
Published on: December 24, 2016
[The place of dendritic cells in the microenviroment in Hodgkin's lymphoma]
Insights
Dendritic cells (DCs) near Hodgkin
Area of Science:
- Immunology and Oncology
- Cell Biology and Pathology
Context:
- Hodgkin's lymphoma (HL) pathogenesis is incompletely understood, particularly the role of microenvironmental elements.
- Dendritic cells (DCs), crucial immune regulators, are understudied in the HL microenvironment.
Purpose:
- To investigate the presence, localization, and morphology of dendritic cells (DCs) within the tumor microenvironment of classical Hodgkin's lymphoma (HL).
- To explore the potential role of specific DC subsets in HL pathogenesis.
Summary:
- Morphological and immunohistochemical analysis of 95 lymph nodes from classical HL patients revealed CD1a- and langerin-positive DCs near neoplastic Reed-Sternberg cells (RSCs) and T lymphocytes.
- Two distinct DC morphologies were observed: mature DCs with networks around RSCs and less mature DCs loosely distributed.
- CD23-positive follicular DCs were found peripherally to tumor foci, associated with residual follicular structures and B lymphocytes, rarely near RSCs.
Impact:
- Identifies specific dendritic cell (DC) subsets and their spatial relationship with Reed-Sternberg cells (RSCs) in Hodgkin's lymphoma (HL).
- Suggests a potential significant role for CD1a- and langerin-positive DCs in HL pathogenesis.
- Highlights the need for further research into the function and precise role of these DCs in HL.
Abstract:
Dendritic cells (DCs) are the least studied microenvironment elements in Hodgkin's lymphoma (HL). Ninety-five lymph nodes were morphologically and immunohistochemically studied in patients with classical HL. The authors found CDla- and langerin-positive DCs in the presence of T lymphocytes, which were localized in the close proximity to neoplastic Reed-Sternberg cells (RSCs). There were also CD23-positive follicular DCs, which were typically located at the periphery of tumor foci, in the areas of residual follicular structures, were surrounded by B lymphocytes and were rarely associated with RSCs. There were two morphologically distinct groups among the CD 1a- and langerin-positive DCs; one had developed cytoplasmic processes, which formed a network around the RSCs (probably a more mature/functionally active form); the other had round cells with short cytoplasmic processes, loosely distributed around the neoplastic cells (a less mature/active form). The function and role of CD1a- and langerin-positive DCs in HL call for further investigation. The topographic closeness of these DCs to RSCs suggests that these cells play a considerable role in the pathogenesis of HL.
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