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Related Concept Videos

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
Experimental RNAi02:15

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...

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Related Experiment Video

Updated: Jun 10, 2026

Generation of Genetically Modified Mice through the Microinjection of Oocytes
10:19

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Published on: June 15, 2017

Transgenic RNAi applications in the mouse.

Jost Seibler1, Frieder Schwenk

  • 1TaconicArtemis GmbH, Cologne, Germany.

Methods in Enzymology
|August 12, 2010
PubMed
Summary
This summary is machine-generated.

RNA interference (RNAi) is a powerful tool for gene function analysis, offering reversible gene silencing and simultaneous multi-gene targeting. This chapter details transgenic RNAi techniques for reverse genetics in mice, including experimental protocols.

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Last Updated: Jun 10, 2026

Generation of Genetically Modified Mice through the Microinjection of Oocytes
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Published on: June 15, 2017

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Loss-of-Function Approach in the Embryonic Chick Retina by Using Tol2 Transposon-Mediated Transgenic Expression of Artificial microRNAs
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Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • RNA interference (RNAi) has become a significant tool in molecular biology over the last decade.
  • Its utility lies in enabling rapid analysis of gene function.
  • Key features include reversible gene silencing and the capacity for simultaneous targeting of multiple genes.

Purpose of the Study:

  • To discuss transgenic RNAi techniques.
  • To provide protocols for applying these techniques in reverse mouse genetics.
  • To highlight the advantages of RNAi for gene function studies.

Main Methods:

  • Detailed explanation of transgenic RNA interference (RNAi) methodologies.
  • Provision of experimental protocols specifically for reverse genetics in mouse models.
  • Focus on techniques enabling gene silencing and functional analysis.

Main Results:

  • Demonstration of RNAi as an effective method for gene function analysis.
  • Illustrations of reversible gene silencing capabilities.
  • Examples of simultaneous multi-gene targeting using RNAi.

Conclusions:

  • Transgenic RNAi is a versatile and powerful approach for genetic research.
  • The provided protocols facilitate the application of RNAi in reverse mouse genetics.
  • RNAi significantly advances the study of gene function and complex genetic pathways.