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Microarray analysis of differentially expressed genes in vaginal tissues in postmenopausal women. The role of stress
Jiali Tong1, Jinghe Lang, Lan Zhu
1Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, People's Republic of China. jialit97@163.com
Introduction And Hypothesis:
This study aimed to explore gene expression and their regulatory mechanisms in etiology of stress urinary incontinence (SUI).
Methods:
Tissue samples were taken from the paraurethral vaginal wall from three pairs of postmenopausal, age/body mass index/parity-matched SUI, and continent women and subjected to microarray analysis using the Affymetrix GeneChip® Human Genome U133 Plus 2.0 oligonucleotide chip set. The significantly differentially expressed genes and their protein expression were confirmed by quantitative PCR and Western blot.
Results:
Seventy-five differentially expressed genes were listed; 31 genes were upregulated in SUI group, while 44 were downregulated. Gene metabolic pathway analysis showed that solutable N-ethylmaleimide-sensitive factor attachment protein receptor signaling pathway and neurodegenerative disorder pathway were the most predominant.
Conclusions:
Apolipoprotein E, growth factor receptor-bound protein 2, Golgi SNAP receptor complex member 1, and glucosidase, beta, acid may play a neurodegenerative role in SUI development.
