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Updated: Jun 10, 2026

Eye Irritation Test (EIT) for Hazard Identification of Eye Irritating Chemicals using Reconstructed Human Cornea-like Epithelial (RhCE) Tissue Model
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An in vitro method for estimating ocular irritation.

R J Soto1, V C Gordon

  • 1S. C. Johnson & Son, Inc., Racine WI and National Testing Corp., Palm Springs, CA, USA.

Toxicology in Vitro : an International Journal Published in Association with BIBRA
|August 13, 2010
PubMed
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The EYTEX in vitro assay (EIA) effectively screens consumer products for irritation potential. The rapid membrane assay (RMA) variant demonstrated the highest accuracy, reducing animal testing and costs.

Area of Science:

  • Toxicology
  • Dermatology
  • In vitro testing

Background:

  • The EYTEX in vitro assay (EIA) is an economical and objective system for evaluating consumer product formulations.
  • Traditional in vivo Draize testing poses ethical concerns and is costly.

Purpose of the Study:

  • To evaluate three variations of the EYTEX in vitro assay (EIA) for their efficacy in predicting in vivo skin irritation.
  • To compare the performance of standard, membrane, and rapid membrane (RMA) EIA methods.

Main Methods:

  • Seventy experimental consumer product formulations were tested using standard, membrane, and RMA EIA methods in two independent laboratories.
  • Results were compared against maximum in vivo Draize scores in a double-blind study.

Main Results:

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Last Updated: Jun 10, 2026

Eye Irritation Test (EIT) for Hazard Identification of Eye Irritating Chemicals using Reconstructed Human Cornea-like Epithelial (RhCE) Tissue Model
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  • The RMA EIA showed the highest correlation with in vivo data and qualified the largest percentage of formulations.
  • RMA demonstrated 89% predictive value for irritants, 84% specificity, and 93% sensitivity.
  • Both laboratories yielded highly correlated results, indicating assay reliability.

Conclusions:

  • The EYTEX in vitro assay (EIA), particularly the RMA variant, is a valuable screening tool for consumer product irritation.
  • EIA offers advantages such as testing undiluted products, reducing animal use, and lowering testing costs.