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Related Concept Videos

Inhibitors of Viral Protein Synthesis01:30

Inhibitors of Viral Protein Synthesis

Protein synthesis is indispensable for viral replication, as viruses lack the cellular machinery required for this process and must hijack the host's translational apparatus. In response, host cells deploy a critical innate immune defense involving interferons, specialized cytokines that play a central role in inhibiting viral propagation.Upon viral detection, infected cells release interferons that bind to receptors on adjacent uninfected cells, activating the JAK-STAT signaling pathway and...
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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
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siRNA - Small Interfering RNAs02:30

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Related Experiment Video

Updated: Jun 10, 2026

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes
10:00

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes

Published on: March 24, 2015

RNA interference and interferon.

Carol A Sledz1, Bryan R G Williams

  • 1Department of Cell Biology, Lerner Research Institute, Cleveland Clinic Foundation, 9500 Euclid Ave., Cleveland, OH 44195, USA.

Discovery Medicine
|August 14, 2010
PubMed
Summary
This summary is machine-generated.

RNA interference (RNAi) uses double-stranded RNA (dsRNA) to silence gene expression, offering therapeutic potential for diseases. However, its use as a research tool faces challenges due to dsRNA

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • RNA interference (RNAi) has evolved from a mysterious cellular response to double-stranded RNA (dsRNA) in organisms like C. elegans.
  • It is now recognized as a potential therapeutic strategy for gene silencing in diseases such as cancer and viral infections.

Purpose of the Study:

  • To explore the progression and application of RNA interference (RNAi) as a research tool.
  • To understand the mechanism of dsRNA recognition and its role in gene silencing.

Main Methods:

  • Recognition and cleavage of foreign dsRNA into short interfering RNAs (siRNAs) by cellular machinery.
  • Incorporation of siRNAs into the RNA Induced Silencing Complex (RISC).

Main Results:

  • dsRNA, a trigger for RNAi, signals cellular danger, originating from foreign sources like viruses or transposons.
  • The RNAi pathway, involving siRNAs and RISC, leads to the degradation of targeted messenger RNA (mRNA).

Conclusions:

  • While RNAi shows therapeutic promise, its application as a research tool is not straightforward.
  • Understanding the dsRNA-mediated RNAi pathway is crucial for harnessing its full potential in research and medicine.