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Curcumin Attenuates PD-L1-Positive Neutrophil-Induced T-Lymphocyte Apoptosis and Alleviates Lung Injury During Sepsis
Jiajun Zou1, Jian Guo1, Guanyu Hu1
1Department of Emergency Medicine, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, 200437 Shanghai, China.
Discovery Medicine
|April 27, 2025
Summary
Curcumin treatment improves survival in septic rats by reducing programmed cell death 1 ligand 1 (PD-L1) expression in neutrophils, thereby decreasing T-lymphocyte apoptosis and mitigating lung injury.
Area of Science:
- Immunology
- Pharmacology
- Pathology
Background:
- Sepsis leads to significant organ damage and high mortality.
- Programmed cell death 1 ligand 1 (PD-L1) plays a role in immune regulation during sepsis.
- Neutrophils are implicated in sepsis-induced T-lymphocyte apoptosis and organ injury.
Purpose of the Study:
- To investigate the effect of curcumin on PD-L1 expression in neutrophils from septic rats.
- To determine curcumin's influence on T-lymphocyte apoptosis and lung injury in a rat sepsis model.
- To explore the in vivo and in vitro mechanisms of curcumin's action.
Main Methods:
- A rat sepsis model was established using cecum ligation and puncture (CLP).
- Curcumin was administered to assess its effects on survival rates, PD-L1 expression, tissue damage, and inflammatory markers.
- Flow cytometry, immunohistochemistry, HE staining, TUNEL assays, MPO activity, and ELISA were employed.
- In vitro studies involved co-culturing neutrophils and T lymphocytes to evaluate apoptosis and PD-1/PD-L1 expression.
Main Results:
- Septic rats had low survival, elevated PD-L1, severe lung/thymus damage, and increased pro-inflammatory cytokines (TNF-α, IL-6).
- Curcumin treatment significantly improved survival, reduced PD-L1 expression, mitigated tissue injury and apoptosis, and modulated cytokine levels.
- In vitro, neutrophils from septic rats induced T-lymphocyte apoptosis and increased PD-1/PD-L1 expression, effects reduced by curcumin and PD-L1 antibodies.
Conclusions:
- Curcumin effectively mitigates lung and thymus injury during sepsis.
- Curcumin attenuates rat T-lymphocyte apoptosis by down-regulating PD-L1 expression.
- These protective effects were observed both in vivo and in vitro.
