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Updated: May 10, 2025

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SPAG4 Regulates Glycolytic Metabolism in HT29 Cells as a Target via the c-MYC/SULT2B1 Pathway

Honghong He1, Xuebing Zhang2, Liting Zhong2

  • 1Radiotherapy Department, Ganzhou Cancer Hospital, 341000 Ganzhou, Jiangxi, China.

Discovery Medicine
|April 27, 2025
PubMed
Abstract

Insights

Sperm-associated antigen 4 (SPAG4) knockdown inhibits HT29 cell proliferation by reducing glycolytic metabolism. This occurs via downregulation of cellular myelocytomatosis oncogene (c-Myc) and sulfotransferase 2B1 (SULT2B1) expression.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Metabolism

Background:

  • Glycolytic metabolism fuels tumor cell proliferation.
  • The SPAG4/c-Myc/SULT2B1 axis role in HT29 cell viability and glycolysis is unexplored.

Purpose of the Study:

  • Investigate SPAG4/c-Myc/SULT2B1 axis regulation of glycolytic metabolism.
  • Determine influence on HT29 cell viability.

Main Methods:

  • Assessed SPAG4, c-Myc, SULT2B1 levels via qRT-PCR and Western blot.
  • Utilized HT29 cell models with overexpression and knockdown.
  • Evaluated cell viability, proliferation, glucose uptake, lactate production, ATP/ADP ratio, GLUT1, and LDHA expression.

Main Results:

  • SPAG4, c-Myc, and SULT2B1 were significantly elevated in HT29 cells.
  • Silencing SPAG4/c-Myc reduced glycolysis, viability, and colony formation.
  • Elevated SULT2B1 counteracted silencing effects, enhancing viability and colony formation.

Conclusions:

  • SPAG4 knockdown suppresses HT29 proliferation and colony formation.
  • This occurs via c-Myc downregulation, reducing SULT2B1 expression and glycolytic metabolism.
Keywords:
cellular myelocytomatosis oncogenecolon cancerglycolysisproliferationsperm-associated antigen 4sulfotransferase 2B1

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