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Related Concept Videos

Hybridoma Technology01:31

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...
Immunological Memory01:23

Immunological Memory

Immunological memory, a pivotal pillar of the adaptive immune system, is responsible for the body's ability to remember and respond more swiftly and effectively to previously encountered pathogens. This remarkable feature is what makes vaccines so effective in preventing diseases.
What is Immunological Memory?
Immunological memory is an integral function of the immune system that allows it to recognize and react more rapidly and effectively to pathogens previously encountered. This feature is...
Vaccine Production01:23

Vaccine Production

Vaccine production involves a sequence of upstream and downstream processes to generate a safe and effective immunological product. It begins with cultivating microorganisms, such as viruses or bacteria, to obtain antigenic material. For viral vaccines, mammalian host cells are grown in bioreactors and subsequently infected with the target virus. The virus replicates within the host cells, which are lysed to release viral particles. This lysate is then clarified through filtration or...
Active versus Passive Immunity01:31

Active versus Passive Immunity

Immunity, along with the ability to limit pathogen growth to prevent significant body tissue damage, can be gained either by (1) actively developing an immune response within the individual after exposure to a pathogen or after getting vaccinated or (2) passively transferring immune components from an immune individual to one who is nonimmune. Both these forms of immunity can be found naturally and in medical practices.
Active Immunity
Active immunity refers to the resistance one develops...
Vaccinations01:51

Vaccinations

Overview
Antibody Structure01:10

Antibody Structure

Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...

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Related Experiment Video

Updated: Jun 10, 2026

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood
13:14

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

Published on: February 6, 2018

Antibody production using genetic immunization.

Ross S Chambers1

  • 1Center for Biomedical Inventions, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75390, USA.

Discovery Medicine
|August 14, 2010
PubMed
Summary
This summary is machine-generated.

Generating proteome-wide antibody sets is crucial for disease biomarker discovery and drug development. Current protein-based antibody production methods are too slow and expensive for the growing needs of proteomics research.

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Last Updated: Jun 10, 2026

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood
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Published on: February 6, 2018

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Area of Science:

  • Proteomics
  • Immunology
  • Biotechnology

Background:

  • Antibodies are essential tools in proteomics, enabling specific detection and quantification of proteins in complex biological samples.
  • High-throughput proteomic studies require comprehensive antibody libraries for biomarker discovery and drug development.
  • Traditional antibody production relies on animal immunization with purified proteins, a process that is time-consuming and costly.

Purpose of the Study:

  • To highlight the critical role of antibodies in quantitative proteomics.
  • To address the limitations of current antibody production methods in meeting the demands of large-scale proteomic research.
  • To emphasize the need for scalable and efficient antibody generation strategies.

Main Methods:

  • Review of traditional antibody production techniques, including protein overexpression and peptide synthesis.
  • Discussion of the impact of genomic sequencing on the discovery of new protein targets.
  • Analysis of the challenges faced by the pharmaceutical and biotechnology industries in antibody generation.

Main Results:

  • Antibodies are indispensable for protein quantification and biomarker identification in proteomics.
  • The rapid discovery of new proteins through genomics has outpaced antibody production capabilities.
  • Current protein-based antibody generation is a bottleneck for quantitative proteomics.

Conclusions:

  • The development of proteome-wide antibody sets is a key objective for advancing disease research and drug discovery.
  • Existing protein-centric antibody production methods are insufficient for the scale required by modern proteomics.
  • Novel, high-throughput antibody generation strategies are urgently needed to support the field of quantitative proteomics.