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Updated: Jun 10, 2026

Drug-induced Sensitization of Adenylyl Cyclase: Assay Streamlining and Miniaturization for Small Molecule and siRNA Screening Applications
Published on: January 27, 2014
[Study on sensitization and mechanism of CGA-BSA]
Xiaowu Huang1, Hongbo Liao, Ping Liu
1Clinical Pharmacology Laboratory, PLA General Hospital, Beijing 100853, China.
Objective:
To investigate the sensitization and mechanism of artificial antigen of chlorogenic acid (CGA-BSA).
Method:
Using intensive immunization to establish allergy animal model on guinea pig and preparing antiserum and tissue for further test. Using HE staining to observe pathology change of lungs, trachea, liver. Using passive mast cell (PMC) degranulation test to observe the immunogenicity of CGA-BSA and using ELISA to detect IgE and histamine in plasma.
Result:
There established allergy animal model on guinea pig, which include a increase cell degranulation by a ratio (63.58 +/- 10.23)% in PMC test, increase of specific antibody IgE and increase of histamine in plasma after provocation by ELISA.
Conclusion:
Allergen CGA-BSA could provoke allergenic response in guinea pig, and the allergic response belongs to type I allergy.
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