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Related Experiment Video

Updated: Jun 10, 2026

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
13:21

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Published on: May 4, 2012

Multidimensional glycan arrays for enhanced antibody profiling.

Yalong Zhang1, Christopher Campbell, Qian Li

  • 1Chemical Biology Laboratory, Center for Cancer Research, NCI-Frederick, 376 Boyles Street, Building 376, Frederick, MD 21702, USA.

Molecular Biosystems
|August 17, 2010
PubMed
Summary

Neoglycoprotein density significantly impacts antibody binding, enhancing glycan array performance for monoclonal antibody development, biomarker discovery, and vaccine optimization by enabling detection of otherwise undetectable immune responses.

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Area of Science:

  • Glycomics and Immunology
  • Carbohydrate-protein interactions
  • Biomarker discovery

Background:

  • Carbohydrate-binding antibodies are crucial in research and clinical settings.
  • Glycan arrays are powerful tools for high-throughput analysis of carbohydrate-protein interactions.
  • Neoglycoprotein arrays offer tunable glycan structure, density, and carrier protein density.

Purpose of the Study:

  • To investigate the effect of neoglycoprotein density on antibody binding.
  • To assess the utility of varied neoglycoprotein densities in profiling serum antibodies.
  • To evaluate the impact of neoglycoprotein density on detecting vaccine-induced immune responses.

Main Methods:

  • Utilized neoglycoprotein arrays with varying densities of blood group A antigen.

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Last Updated: Jun 10, 2026

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
13:21

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Published on: May 4, 2012

Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling
11:08

Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling

Published on: February 5, 2018

Profiling Anti-Neu5Gc IgG in Human Sera with a Sialoglycan Microarray Assay
09:13

Profiling Anti-Neu5Gc IgG in Human Sera with a Sialoglycan Microarray Assay

Published on: July 13, 2017

  • Evaluated binding of five monoclonal antibodies to the arrays.
  • Profiled serum antibodies from healthy individuals and responses to a prostate cancer vaccine.

    • Main Results:

    • Neoglycoprotein density substantially affected monoclonal antibody recognition of the blood group A antigen.
    • Multiple neoglycoprotein densities aided in distinguishing antibody subpopulations in healthy individuals.
    • Variations in neoglycoprotein density enabled detection of previously undetectable vaccine-induced antibody responses.

    Conclusions:

    • Neoglycoprotein density is a critical factor in antibody recognition.
    • Multidimensional glycan arrays incorporating varied neoglycoprotein densities enhance performance for antibody development and biomarker discovery.
    • Optimizing neoglycoprotein density is key for vaccine development and immune response evaluation.