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Soluble human lymphocyte sugar binding proteins with immunosuppressive activity.
C Grillon1, M Monsigny, C Kieda
1Département de Biochimie des Glycoconjugués et Lectines Endogènes, Centre de Biophysique Moléculaire, CNRS, Orléans, France.
Immunology Letters
|April 1, 1991
Summary
Alpha-L-rhamnose inhibits immune suppression. Researchers identified rhamnose-specific receptors on human CD8+ T cells and isolated novel rhamnose-binding fractions (RBF) that suppress lymphocyte DNA synthesis and B cell immunoglobulin production.
Area of Science:
- Immunology
- Glycobiology
Background:
- Immune response suppression involves T cells and suppressor factors.
- Alpha-L-rhamnose is known to inhibit this immune suppression.
Purpose of the Study:
- To identify rhamnose-specific receptors on human CD8+ T cells.
- To isolate and characterize novel molecules involved in rhamnose-mediated immune regulation.
Main Methods:
- Isolation of rhamnose-specific molecules (RBF) from lymphocyte extracts.
- Purification of RBF using affinity chromatography.
- Assessment of RBF's ability to bind rhamnose via fluorescence labeling.
- Evaluation of RBF's effect on mitogen-stimulated human lymphocyte DNA synthesis and B cell immunoglobulin production.
Main Results:
- Demonstrated the presence of rhamnose-specific receptors on human CD8+ T cells.
- Successfully isolated and purified rhamnose-binding fractions (RBF).
- Confirmed RBF's ability to bind alpha-L-rhamnose.
- Showed that RBF efficiently suppresses human lymphocyte DNA synthesis and B cell immunoglobulin production.
Conclusions:
- Identified antigen-independent suppressor factors that bind alpha-L-rhamnose.
- These findings suggest a novel mechanism for immune regulation involving specific sugar-binding molecules on T cells.