Related Experiment Video

Updated: Jun 10, 2026

Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease
04:41

Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease

Published on: July 3, 2018

Cell free DNA detected by a novel method in acute ST-elevation myocardial infarction patients

Avi Shimony1, Doron Zahger, Harel Gilutz

  • 1Department of Cardiology, Soroka University Medical Center, Ben Gurion University of the Negev, Beer Sheva, Israel. ashimony@bgu.ac.il;

Acute Cardiac Care
|August 18, 2010
PubMed

Insights

Circulating cell-free DNA (CFD) levels are elevated in ST-elevation myocardial infarction (STEMI) patients. This novel assay shows CFD correlates with cardiac necrosis markers but not ventricular function, warranting further study.

Area of Science:

  • Cardiology
  • Biochemistry
  • Molecular Diagnostics

Background:

  • Elevated circulating cell-free DNA (CFD) indicates poor prognosis in various diseases.
  • Data on CFD in acute myocardial infarction (MI) is limited, with existing detection methods being cumbersome.
  • A novel, rapid fluorometric assay for CFD detection was investigated.

Purpose of the Study:

  • To evaluate a novel method for detecting CFD in ST-elevation myocardial infarction (STEMI) patients.
  • To assess the correlation between CFD levels and established markers of myocardial necrosis.
  • To examine the relationship between CFD and ventricular function.

Main Methods:

  • Serum CFD, troponin-T, and creatine kinase (CK) were measured in 16 STEMI patients and 47 controls.
  • CFD was quantified using a novel rapid fluorometric assay.
  • Ejection fraction (EF) was assessed via echocardiography.

Main Results:

  • Peak CFD levels were significantly higher in STEMI patients versus controls (P=0.001).
  • Peak CFD levels correlated strongly with peak CK (R=0.79) and troponin-T (R=0.65) levels.
  • A trend suggested an association between peak CFD and lower ejection fraction (P=0.075).

Conclusions:

  • The novel CFD assay effectively correlates with myocardial necrosis markers in STEMI.
  • CFD levels did not significantly correlate with ejection fraction in this study.
  • Further research is needed to understand CFD kinetics and prognostic value post-STEMI.
Abstract

More Related Videos

A Research Method For Detecting Transient Myocardial Ischemia In Patients With Suspected Acute Coronary Syndrome Using Continuous ST-segment Analysis18:11

A Research Method For Detecting Transient Myocardial Ischemia In Patients With Suspected Acute Coronary Syndrome Using Continuous ST-segment Analysis

Published on: December 28, 2012

A Sensitive and Specific Quantitation Method for Determination of Serum Cardiac Myosin Binding Protein-C by Electrochemiluminescence Immunoassay10:12

A Sensitive and Specific Quantitation Method for Determination of Serum Cardiac Myosin Binding Protein-C by Electrochemiluminescence Immunoassay

Published on: August 8, 2013

Related Concept Videos

Acute Coronary Syndrome III: Diagnostic Studies01:30

Acute Coronary Syndrome III: Diagnostic Studies

Diagnosing acute coronary syndrome or ACS begins with a thorough patient history. Notable symptoms include central, crushing chest pain radiating to the left arm, neck, jaw, or back, along with shortness of breath, sweating (diaphoresis), nausea, vomiting, dizziness, and palpitations.It is crucial to note any history of cardiac illnesses and assess risk factors, including age, gender, smoking, hypertension, diabetes, hyperlipidemia, and a sedentary lifestyle.During physical examination, vital...
Blood Studies for Cardiovascular System I: Cardiac Biomarkers01:20

Blood Studies for Cardiovascular System I: Cardiac Biomarkers

Cardiac biomarkers are enzymes, proteins, and hormones released into the blood when cardiac cells are injured. They are powerful tools for triaging.
The essential diagnostic tools for detecting myocardial necrosis and monitoring individuals suspected of having acute coronary syndrome (ACS) include:
Troponins
Troponins, particularly cardiac troponins I and T, are the most precise and sensitive markers of myocardial injury. They are detectable within 4-6 hours of myocardial injury and remain...
Acute Coronary Syndrome I: Introduction01:30

Acute Coronary Syndrome I: Introduction

Acute Coronary Syndrome (ACS) encompasses a spectrum of heart conditions caused by sudden obstruction of coronary arteries, typically resulting from the rupture of an atherosclerotic plaque and subsequent thrombus (blood clot) formation. This obstruction can lead to partial or complete blockage of blood flow, causing varying degrees of myocardial ischemia or infarction.ACS includes the following clinical entities:Unstable Angina (UA)Non-ST-Elevation Myocardial Infarction (NSTEMI)ST-Elevation...
Acute Coronary Syndrome II: Pathophysiology and Clinical Manifestations01:19

Acute Coronary Syndrome II: Pathophysiology and Clinical Manifestations

The pathophysiology of Acute Coronary Syndrome [ACD] involves several key processes:The main underlying cause of ACD is atherosclerosis, a chronic inflammatory disease characterized by the buildup of lipid-laden plaques within the coronary arteries.As the atherosclerotic plaque grows in the coronary artery, it may become unstable due to the formation of a lipid-rich core and a thin fibrous cap. Inflammatory cells within the plaque, such as macrophages, secrete enzymes that degrade the...
Myocarditis II: Clinical Features and Diagnostic Tests01:27

Myocarditis II: Clinical Features and Diagnostic Tests

Myocarditis is an inflammation of the heart muscle. The symptoms vary widely, encompassing asymptomatic presentations to severe, acute manifestations.Clinical PresentationAsymptomatic cases: In some instances, myocarditis may be asymptomatic, with the infection resolving without intervention. These cases often go undetected unless discovered incidentally through diagnostic imaging or tests conducted for other reasons.General Early Symptoms: Early symptoms of myocarditis are non-specific and can...