Detection of bisphenol A using a novel surface plasmon resonance biosensor

Area of Science:

• Analytical Chemistry
• Environmental Science
• Biotechnology

Background:

• Bisphenol A (BpA) is an endocrine-disrupting chemical with widespread environmental presence.
• Accurate and sensitive detection methods for BpA are crucial for environmental monitoring and public health.
• Existing detection methods like ELISA and HPLC have limitations in speed, cost, or portability.

Purpose of the Study:

• To develop and validate a compact surface plasmon resonance (SPR) biosensor for sensitive BpA detection.
• To evaluate the biosensor's performance in different matrices, including phosphate-buffered saline (PBS) and wastewater.
• To assess the biosensor's reusability and compare its efficacy with conventional analytical techniques.

Main Methods:

• Utilized a surface plasmon resonance sensor platform (SPRCD) in a binding inhibition format.
• Performed quantitative detection of BpA in PBS and wastewater samples.
• Determined the limit of detection (LOD) and assessed the biosensor's regeneration capabilities.

Main Results:

• The SPR biosensor demonstrated sensitive detection of BpA across a concentration range of 0.05 to 1,000 ng/ml.
• The limit of detection for BpA was determined to be 0.08 ng/ml in PBS and 0.14 ng/ml in wastewater.
• The biosensor proved to be regenerable for multiple detection cycles, indicating its practical utility.

Conclusions:

• The developed compact SPR biosensor is a highly sensitive and reusable platform for BpA detection.
• The biosensor shows promise for environmental monitoring applications, offering comparable or superior performance to ELISA and HPLC.
• This technology provides a valuable tool for assessing BpA contamination in complex samples like wastewater.