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Tagging and Fusion Proteins

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...

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Related Experiment Video

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Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

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Published on: June 23, 2022

A cleavable linker based on the levulinoyl ester for activity-based protein profiling

Paul P Geurink¹, Bogdan I Florea, Nan Li

¹Leiden Institute of Chemistry and The Netherlands Proteomics Center, Leiden University, Einsteinweg 55, 2300 RA, Leiden, The Netherlands.

Angewandte Chemie (International Ed. in English)

|August 18, 2010

Summary

No abstract available in PubMed .

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