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Selective determination of altertoxins by high-performance liquid chromatography with electrochemical detection with
A Visconti1, A Sibilia, F Palmisano
1Istituto Tossine e Micotossine da Parassiti Vegetali, Consiglio Nazionale delle Ricerche, Via G. Amendola, Bari, Italy.
Abstract:
A selective method for the determination of altertoxin-I and altertoxin-II by high-performance liquid chromatography with electrochemical detection is described. Altertoxins were separated on a reversed-phase column with methanol-water containing 0.1 M sodium nitrate and 1 mM nitric acid (60:40) as eluent and detected with dual in-series electrodes operating in the "redox" mode (generator electrode +1.0 V, indicator electrode -0.1 V). The method was applied successfully to the determination of sub-ppm levels of altertoxins in samples of maize, rice and tomatoes infected by Alternaria alternata.