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Related Concept Videos

CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

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Related Experiment Video

Updated: Jun 10, 2026

Genome Editing with CompoZr Custom Zinc Finger Nucleases (ZFNs)
09:11

Genome Editing with CompoZr Custom Zinc Finger Nucleases (ZFNs)

Published on: June 14, 2012

Genome editing with engineered zinc finger nucleases.

Fyodor D Urnov1, Edward J Rebar, Michael C Holmes

  • 1Sangamo BioSciences Inc., Richmond, California 94804, USA.

Nature Reviews. Genetics
|August 19, 2010
PubMed
Summary

Genome editing utilizes engineered zinc finger nucleases for targeted DNA cleavage and gene modification. This powerful technology enables advancements in model organisms, human cells, and crop plants, opening new therapeutic avenues.

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Last Updated: Jun 10, 2026

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Published on: June 14, 2012

Mouse Genome Engineering Using Designer Nucleases
12:04

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Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation
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Published on: February 2, 2016

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Reverse genetics and genome engineering are crucial for biological research.
  • Zinc finger nucleases (ZFNs) offer sequence-specific DNA targeting capabilities.

Purpose of the Study:

  • To highlight the broad applications of targeted genome cleavage using engineered zinc finger nucleases.
  • To underscore the establishment of genome editing in various cell types and organisms.

Main Methods:

  • Application of engineered, sequence-specific zinc finger nucleases for targeted genome cleavage.
  • Facilitation of gene modification through cellular repair mechanisms following cleavage.

Main Results:

  • Successful genome engineering in diverse model organisms (Drosophila, zebrafish, rats) and plants (Arabidopsis).
  • Demonstrated efficiency in human embryonic and induced pluripotent stem cells.
  • Achieved targeted integration in crop plants and HIV resistance in immune cells.

Conclusions:

  • Genome editing via zinc finger nucleases is a versatile and established technology.
  • This technology opens significant new experimental and therapeutic possibilities across various biological systems.