Related Experiment Video
Updated: Jun 10, 2026
06:58
Collection and Extraction of Saliva DNA for Next Generation Sequencing
Published on: August 27, 2014
Collection, storage, and processing of saliva samples for downstream molecular applications
Bradley Stephen Henson1, David T Wong
1College of Dental Medicine, Western University of Health Sciences, Pomona, CA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|August 19, 2010
Summary
Saliva analysis offers a powerful, non-invasive method for detecting various oral and systemic diseases using molecular biomarkers. This research details optimal saliva collection, processing, and storage protocols for accurate diagnostic testing.
Area of Science:
- Biomarker Discovery
- Translational Research
- Oral Health Diagnostics
Background:
- Saliva is a valuable diagnostic medium for oral and systemic diseases.
- Advancements in technology enable large-scale, high-throughput salivary biomarker studies.
- Detecting diseases like cancer, hepatitis, and HIV through saliva is a growing area of research.
Purpose of the Study:
- To highlight the potential of saliva as a diagnostic tool.
- To describe established protocols for saliva collection, processing, and storage.
- To facilitate the discovery and validation of salivary disease biomarkers.
Main Methods:
- Review of current literature on saliva-based diagnostics.
- Description of standardized procedures for saliva sample handling.
- Emphasis on optimizing pre-analytical conditions for molecular analysis.
Main Results:
- Saliva analysis can identify biomarkers for caries risk, periodontitis, and various cancers.
- Systemic conditions such as hepatitis, HIV, and HCV can be detected in saliva.
- Proper sample handling is crucial for reliable and reproducible results.
Conclusions:
- Saliva is an accessible and effective medium for molecular diagnostics.
- Standardized protocols are essential for accurate salivary biomarker analysis.
- Further research in salivary diagnostics holds promise for improved health monitoring.
Related Concept Videos
01:23Salivary Glands and Saliva
The salivary glands, of which there are three pairs known as the parotid, submandibular, and sublingual glands, play a crucial role in maintaining oral health and initiating the digestive process. Positioned near the ears, beneath the masseter muscle, the parotid glands secrete saliva into the oral cavity through the parotid duct of Stensen. Meanwhile, the submandibular glands, located on the floor of the mouth, secrete saliva through channels named submandibular ducts. The sublingual glands,...
01:02Sample Handling
Transportation of samples from the collection point to the laboratory, as well as storage and preservation techniques, are crucial for maintaining sample integrity and ensuring accurate and reliable test results.
Samples should be transported carefully from collection points to the laboratory. They should be properly sealed and clearly labeled to prevent cross-contamination. To preserve the sample integrity, optimal temperature conditions during transport are essential. This could involve using...
Samples should be transported carefully from collection points to the laboratory. They should be properly sealed and clearly labeled to prevent cross-contamination. To preserve the sample integrity, optimal temperature conditions during transport are essential. This could involve using...