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Related Concept Videos

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

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Related Experiment Video

Updated: Jun 10, 2026

Collection and Extraction of Saliva DNA for Next Generation Sequencing
06:58

Collection and Extraction of Saliva DNA for Next Generation Sequencing

Published on: August 27, 2014

Transcriptomic analyses of saliva.

Viswanathan Palanisamy1, David T Wong

  • 1College of Dental Medicine, Medical University of South Carolina, Charleston, SC, USA.

Methods in Molecular Biology (Clifton, N.J.)
|August 19, 2010
PubMed
Summary
This summary is machine-generated.

Salivary mRNA analysis offers a novel, noninvasive method for disease detection. These advanced techniques identify thousands of mRNA species in saliva, paving the way for new diagnostic biomarkers.

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Efficient SARS-CoV-2 Quantitative Reverse Transcriptase PCR Saliva Diagnostic Strategy utilizing Open-Source Pipetting Robots
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Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles
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Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles

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Last Updated: Jun 10, 2026

Collection and Extraction of Saliva DNA for Next Generation Sequencing
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Collection and Extraction of Saliva DNA for Next Generation Sequencing

Published on: August 27, 2014

Efficient SARS-CoV-2 Quantitative Reverse Transcriptase PCR Saliva Diagnostic Strategy utilizing Open-Source Pipetting Robots
11:11

Efficient SARS-CoV-2 Quantitative Reverse Transcriptase PCR Saliva Diagnostic Strategy utilizing Open-Source Pipetting Robots

Published on: February 11, 2022

Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles
08:50

Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles

Published on: October 10, 2013

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genomics

Background:

  • Salivary biomarkers are increasingly recognized for diagnostic and prognostic applications.
  • Noninvasive molecular diagnostics are a rapidly growing field.
  • Salivary mRNA transcriptomic analysis presents a new paradigm for disease detection.

Purpose of the Study:

  • To overview the development of sensitive and robust assays for mRNA biomarker discovery and validation in human saliva.
  • To highlight the utility of microarray and quantitative reverse transcriptase-PCR (qRT-PCR) methods.

Main Methods:

  • Microarray profiling using Human Genome U133 Plus 2.0 and Exon 1.0 ST array platforms.
  • RNA linear amplification from nanogram quantities.
  • Enzymatic cleanup and validation by quantitative PCR.
  • RNA integrity analysis using Agilent Bioanalyzer and quantification via Nanodrop spectrophotometer.

Main Results:

  • Identification of thousands of mRNA species in saliva.
  • Demonstration of sensitive and robust assay development.
  • Validation of salivary mRNA as a medium for biomarker discovery.

Conclusions:

  • Salivary mRNA transcriptomic analysis is an efficient approach for biomarker discovery.
  • This method facilitates the detection of oral and systemic diseases.
  • The developed techniques support noninvasive molecular diagnostics.