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Imaging cilia in zebrafish.

Kimberly M Jaffe¹, Stephan Y Thiberge, Margaret E Bisher

¹Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA.

Methods in Cell Biology

|August 20, 2010

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This study details methods for imaging cilia, crucial cellular organelles, in zebrafish embryos. Understanding cilia is vital for human health, as defects cause various diseases.

Area of Science:

Cell Biology
Developmental Biology
Genetics

Background:

Cilia are vital cellular organelles implicated in numerous human diseases when dysfunctional.
Research into cilia structure, regulation, and function is rapidly expanding.
Zebrafish (Danio rerio) serve as an excellent model organism for studying cilia.

Purpose of the Study:

To describe established and novel methods for visualizing cilia and their motility in zebrafish embryos.
To provide a comprehensive guide for researchers studying cilia in a vertebrate model system.

Main Methods:

Whole-mount immunofluorescence staining for cilia visualization.
Transverse cryosectioning and immunohistochemistry for detailed structural analysis.
Transmission electron microscopy (TEM) for ultrastructural examination of cilia.

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Published on: February 19, 2016

Related Experiment Videos

Last Updated: Jun 10, 2026

Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence

Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence

Published on: November 18, 2017

Labeling and Imaging Cells in the Zebrafish Hindbrain

Labeling and Imaging Cells in the Zebrafish Hindbrain

Published on: July 25, 2010

Immunostaining Phospho-epitopes in Ciliated Organs of Whole Mount Zebrafish Embryos

Immunostaining Phospho-epitopes in Ciliated Organs of Whole Mount Zebrafish Embryos

Published on: February 19, 2016

Live imaging of cilia motility in embryonic structures like Kupffer's vesicle and pronephros.

Main Results:

Detailed protocols for multiple imaging techniques are presented.
The study demonstrates the feasibility of imaging cilia and their motility in various zebrafish embryonic tissues.
The described methods allow for both static and dynamic analysis of cilia.

Conclusions:

The presented imaging techniques are effective for studying cilia structure and function in zebrafish.
This work provides valuable resources for the scientific community investigating cilia-related biology and disease.
Zebrafish offer a powerful platform for advancing our understanding of cilia in health and disease.