Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Quantitative nuclear-cytoplasmic ratios in human hepatomas.

Archives of Pathology & Laboratory Medicine

|April 1, 1978

Summary

Quantitative analysis of nuclear-cytoplasmic (N/C) ratios in liver cells can aid diagnosis. Hepatoma cells show higher N/C ratios than normal or cirrhotic liver cells, with greater variance.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Reference range for 24-h urine calcium, calcium/creatinine ratio, and correlations with calcium absorption and serum vitamin D metabolites in normal women.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2020

Same author

Effect of vitamin D supplementation on total and free 25 hydroxyvitamin D and parathyroid hormone. An analysis of two randomized controlled trials.

Journal of internal medicine·2019

Same author

Effect of increasing doses of vitamin D on bone mineral density and serum N-terminal telopeptide in elderly women: a randomized controlled trial.

Journal of internal medicine·2018

Same author

Robust Zero-Field Skyrmion Formation in FeGe Epitaxial Thin Films.

Physical review letters·2017

Same author

Epitaxial growth of iridate pyrochlore Nd2Ir2O7 films.

Scientific reports·2016

Same author

Treatment with denosumab reduces secondary fracture risk in women with postmenopausal osteoporosis.

Climacteric : the journal of the International Menopause Society·2015

Area of Science:

Hepatology
Cell Biology
Diagnostic Pathology

Background:

Accurate differentiation between neoplastic and non-neoplastic liver cells is crucial for patient management.
Nuclear-cytoplasmic (N/C) ratio is a recognized morphological parameter in cell characterization.
Existing methods for N/C ratio assessment may lack quantitative precision.

Purpose of the Study:

To quantitatively assess nuclear-cytoplasmic (N/C) ratios in human hepatoma, normal liver, and cirrhotic liver specimens.
To determine if N/C ratios can serve as a reliable diagnostic marker for hepatocellular carcinoma.
To compare the variance of N/C ratios between cancerous and non-cancerous liver cells.

Main Methods:

Quantitative morphometric analysis using point-counting techniques.
Utilized an intraocular microscopic grid for precise point sampling.
Specimens included human hepatomas, normal liver tissue, and cirrhotic liver tissue.

Main Results:

Hepatoma cells predominantly exhibited N/C ratios greater than 0.21.
Normal and cirrhotic liver cells primarily showed N/C ratios less than 0.21.
A statistically significant greater variance in N/C ratios was observed in hepatoma cells compared to normal and cirrhotic cells.

Conclusions:

Quantitative N/C ratios provide valuable data for distinguishing hepatoma cells from normal and cirrhotic liver cells.
The distinct N/C ratio profiles suggest potential utility in the histopathological diagnosis of hepatocellular carcinoma.
This quantitative approach offers an objective method for cell characterization in liver pathology.

Related Experiment Videos