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Updated: Jun 10, 2026

A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells
Published on: July 3, 2013
Targeted messenger RNA profiling of transfected breast cancer gene in a living cell
D Nawarathna1, R Chang, E Nelson
1Department of Electrical Engineering and Computer Science, University of California, Irvine, Irvine, CA 92697, USA.
Abstract:
Selective messenger RNA (mRNA) profiling of transfected breast cancer gene expression in a living cell is demonstrated. Atomic force microscope (AFM) probe tips are structurally modified to create a dielectrophoretic force that attracts mRNA molecules within the cell nucleus. The tip end is chemically modified to hybridize only to the target mRNA from a pool of molecules within the nucleus. We successfully combined this scheme with standard assay techniques to develop an assay technology that can be used for early disease detection and basic studies in cell biology.
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