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Related Experiment Video

Updated: Jun 10, 2026

Imaging Denatured Collagen Strands In vivo and Ex vivo via Photo-triggered Hybridization of Caged Collagen Mimetic Peptides
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Imaging Denatured Collagen Strands In vivo and Ex vivo via Photo-triggered Hybridization of Caged Collagen Mimetic Peptides

Published on: January 31, 2014

Collagen targeting using multivalent protein-functionalized dendrimers.

Monica Breurken1, Edith H M Lempens, Rinske P Temming

  • 1Laboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of Technology, PO Box 513, 5600 MB Eindhoven, The Netherlands.

Bioorganic & Medicinal Chemistry
|August 21, 2010
PubMed
Summary
This summary is machine-generated.

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Researchers developed protein dendrimers to target collagen, a key marker in tissue remodeling diseases. These multivalent ligands significantly enhanced collagen binding affinity and imaging capabilities.

Area of Science:

  • Biomaterials Science
  • Protein Engineering
  • Molecular Imaging

Background:

  • Collagen is a crucial biomarker for tissue remodeling in various diseases.
  • Developing targeted ligands for collagen is essential for diagnostic and therapeutic applications.

Purpose of the Study:

  • To engineer protein dendrimers as multivalent ligands for enhanced collagen targeting.
  • To investigate the impact of multivalent display and target density on collagen binding affinity.

Main Methods:

  • Native chemical ligation was used to conjugate the collagen-binding protein CNA35 to dendritic wedges.
  • Binding affinities were assessed on collagen-immobilized surfaces and native collagen in rat intestinal tissues.
  • Collagen-mimicking surfaces with varying peptide densities were created to study target density effects.

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Recombinant Collagen I Peptide Microcarriers for Cell Expansion and Their Potential Use As Cell Delivery System in a Bioreactor Model
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Recombinant Collagen I Peptide Microcarriers for Cell Expansion and Their Potential Use As Cell Delivery System in a Bioreactor Model

Published on: February 7, 2018

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Last Updated: Jun 10, 2026

Imaging Denatured Collagen Strands In vivo and Ex vivo via Photo-triggered Hybridization of Caged Collagen Mimetic Peptides
07:03

Imaging Denatured Collagen Strands In vivo and Ex vivo via Photo-triggered Hybridization of Caged Collagen Mimetic Peptides

Published on: January 31, 2014

Recombinant Collagen I Peptide Microcarriers for Cell Expansion and Their Potential Use As Cell Delivery System in a Bioreactor Model
08:43

Recombinant Collagen I Peptide Microcarriers for Cell Expansion and Their Potential Use As Cell Delivery System in a Bioreactor Model

Published on: February 7, 2018

Main Results:

  • Multivalent display of a weak-binding CNA35 variant significantly increased collagen affinity.
  • The protein dendrimers demonstrated restored collagen imaging capacities, particularly the tetravalent system.
  • Dissociation of the multivalent dendrimers from collagen surfaces was substantially reduced.

Conclusions:

  • Protein dendrimers effectively enhance collagen targeting by leveraging multivalent interactions.
  • These engineered ligands show promise for improved collagen-based imaging and diagnostics in disease.
  • The study highlights the importance of multivalent display for overcoming weak individual binding interactions.