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Related Concept Videos

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...
NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...

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Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
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Published on: May 18, 2011

Confocal Raman microscopy: common errors and artefacts.

Neil J Everall1

  • 1Intertek-MSG, Wilton Research Centre, Wilton, Redcar TS104RF, UK. Neil.Everall@intertek.com

The Analyst
|August 21, 2010
PubMed
Summary
This summary is machine-generated.

Confocal Raman microscopy offers powerful non-destructive depth profiling for transparent materials. Optimizing instrument configuration minimizes spectral noise and improves spatial resolution for accurate chemical analysis.

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Area of Science:

  • Chemical Sciences
  • Materials Science
  • Life Sciences

Background:

  • Confocal Raman microscopy is vital for non-destructive depth profiling in various scientific fields.
  • Existing Raman microscope configurations often lead to suboptimal performance, including low signal-to-noise ratios and inaccurate depth measurements.

Purpose of the Study:

  • To review common aberrations and artifacts in confocal Raman microscopy.
  • To provide guidance on optimizing Raman microscope configurations for improved depth profiling.

Main Methods:

  • Discussion of optical principles and common pitfalls in Raman microscopy setup.
  • Analysis of factors affecting signal-to-noise ratio, spatial resolution, and depth accuracy.

Main Results:

  • Identification of specific aberrations and artifacts that degrade spectral quality and depth profiling accuracy.
  • Principles for optimal optical configuration are presented to mitigate these issues.

Conclusions:

  • Adherence to fundamental optical microscopy principles can significantly enhance confocal Raman microscopy performance.
  • Improved configurations lead to more reliable and accurate depth profiling for chemical and materials analysis.