Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 10, 2026

Isolation of Murine Peritoneal Macrophages to Carry Out Gene Expression Analysis Upon Toll-like Receptors Stimulation
08:21

Isolation of Murine Peritoneal Macrophages to Carry Out Gene Expression Analysis Upon Toll-like Receptors Stimulation

Published on: April 29, 2015

Gene expression reprogramming protects macrophage from septic-induced cell death.

Edielle Sant'Anna Melo1, Denise F Barbeiro, Renata Gorjão

  • 1Emergency Medicine Department, Medical School, University of São Paulo, São Paulo, Brazil.

Molecular Immunology
|August 24, 2010
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dietary restriction and life-history trade-offs: insights into mTOR pathway regulation and reproductive investment in Japanese quail.

The Journal of experimental biology·2024
Same author

Neurobehavioral dysfunction in a mouse model of Down syndrome: upregulation of cystathionine β-synthase, H<sub>2</sub>S overproduction, altered protein persulfidation, synaptic dysfunction, endoplasmic reticulum stress, and autophagy.

GeroScience·2024
Same author

Embryonic methionine triggers post-natal developmental programming in Japanese quail.

Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology·2024
Same author

In ovo feeding of methionine affects antioxidant status and growth-related gene expression of TETRA SL and Hungarian indigenous chicks.

Scientific reports·2024
Same author

Methionine sources and genotype affect embryonic intestinal development, antioxidants, tight junctions, and growth-related gene expression in chickens.

Animal nutrition (Zhongguo xu mu shou yi xue hui)·2024
Same author

Calming Hungarian Grey Cattle in Headlocks Using Processed Nasal Vocalization of a Mother Cow.

Animals : an open access journal from MDPI·2024

Lipopolysaccharide (LPS) tolerance in mice alters gene expression, reducing inflammatory responses and cell death. This immune reprogramming enhances survival rates during polymicrobial sepsis.

Area of Science:

  • Immunology
  • Molecular Biology
  • Sepsis Research

Background:

  • Sepsis triggers systemic inflammation, leading to tissue damage and cell death.
  • Lipopolysaccharide (LPS) tolerance influences the inflammatory response.
  • Understanding immune regulation during endotoxemia is crucial.

Purpose of the Study:

  • To investigate macrophage mRNA expression changes induced by LPS tolerance.
  • To identify novel mechanisms of immune regulation in endotoxemia.
  • To link gene expression reprogramming to protection against sepsis-induced cell death and mortality.

Main Methods:

  • LPS tolerance induced via daily subcutaneous LPS administration over 5 days.
  • Quantitative macroarray analysis of 1200 genes in spleen-derived macrophages.

More Related Videos

Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages
12:47

Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages

Published on: November 3, 2014

Related Experiment Videos

Last Updated: Jun 10, 2026

Isolation of Murine Peritoneal Macrophages to Carry Out Gene Expression Analysis Upon Toll-like Receptors Stimulation
08:21

Isolation of Murine Peritoneal Macrophages to Carry Out Gene Expression Analysis Upon Toll-like Receptors Stimulation

Published on: April 29, 2015

Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages
12:47

Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages

Published on: November 3, 2014

  • Validation of key gene expressions (PARP-1, caspase 3, FASL, TRAIL, TNF-α, IFN-γ) using RT-PCR.
  • Flow cytometry to assess macrophage necrosis and apoptosis.
  • Survival studies in a polymicrobial sepsis model.

    • Main Results:

    • LPS tolerance altered the expression of 84 mRNAs in macrophages compared to naïve mice.
    • Significant downregulation of pro-apoptotic genes (PARP-1, caspase 3, FASL, TRAIL) and pro-inflammatory cytokines (TNF-α, IFN-γ) was observed.
    • Tolerant macrophages exhibited reduced necrosis and apoptosis following sepsis challenge.
    • LPS-tolerant mice showed significantly lower mortality rates.

    Conclusions:

    • LPS tolerance selectively reprograms gene expression pathways related to cell death.
    • This reprogramming confers protection against sepsis-induced macrophage death.
    • LPS tolerance enhances survival rates in a polymicrobial sepsis model, highlighting a potential therapeutic strategy.