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Updated: Jun 10, 2026

Capillary-based Centrifugal Microfluidic Device for Size-controllable Formation of Monodisperse Microdroplets

Capillary-based Centrifugal Microfluidic Device for Size-controllable Formation of Monodisperse Microdroplets

Published on: February 22, 2016

A continuous-flow, microfluidic fraction collection device.

Christopher A Baker¹, Michael G Roper

¹Department of Chemistry and Biochemistry, Florida State University, Tallahassee, 32306, USA.

Journal of Chromatography. A

|August 24, 2010

Summary

This summary is machine-generated.

This study introduces a microfluidic device for electrophoretic separation and fraction collection. Optimized geometry ensures efficient sample collection with minimal carryover, advancing automated microfluidic applications.

Keywords:

electrophoresis fraction collection microfluidic separation

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Area of Science:

Analytical Chemistry
Microfluidics
Biotechnology

Background:

Microfluidic devices offer miniaturized platforms for complex chemical and biological analyses.
Automated sample processing and collection are crucial for high-throughput screening and precious sample preservation.

Purpose of the Study:

To develop and optimize a microfluidic device capable of performing electrophoretic separation coupled with on-chip fraction collection.
To enable precise control over sample fractionation for automated or continuous microfluidic operations.

Main Methods:

Electrophoretic separation within a 3.5 cm channel.
Sheath flow focusing to direct effluent into one of seven collection channels.
Optimization of device geometry using a three-level factorial model to minimize carryover.

Main Results:

Successful separation and collection of a fluorescently-labeled amino acid mixture.
Achieved precise effluent direction by varying sheath flow channel voltage ratios.
Optimized channel angles (22.5°) and lengths (350 µm) for efficient fractionation.

Conclusions:

The developed microfluidic device effectively integrates electrophoretic separation with fraction collection.
Optimized geometry minimizes carryover, enhancing the utility of microfluidic platforms for automated and precious sample handling.