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Updated: Jun 10, 2026

Three-Dimensionally Printed Microfluidic Cross-flow System for Ultrafiltration/Nanofiltration Membrane Performance Testing
Published on: February 13, 2016
Inline ultrafiltration
Christopher A Teske1, Benedicte Lebreton, Robert van Reis
1Late Stage Purification Process R&D, Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, USA. cteske@gene.com
Abstract:
Inline ultrafiltration (UF) can significantly increase the recoverable mass of biopharmaceutical products when pool tank volumes are limiting. Using relatively small commercially available ultrafiltration cassettes, a proof-of-concept study demonstrates that inline UF can significantly increase recoverable mass in an antibody purification process. With ever-increasing cell culture titers pushing product masses to higher levels, inline UF offers a relatively easy-to-implement and less disruptive alternative to installing larger pool tanks and enables more cost-effective production utilizing facilities designed for smaller bulk sizes.
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