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Updated: Jun 10, 2026
Quantification of Tumor Cell Adhesion in Lymph Node Cryosections
Published on: February 9, 2020
[The value and association of CCR7 expression in NSCLC with lymph node metastasis.]
Tao Zeng1, Jianhu Wen, Xing Li
1Department of Cardiothoracic Surgery,Mianyang 404 Hospital, Mianyang, Sichuan 621000, China. nhzengtao@yahoo.com.cn.
Background:
It has became a hotspot research about the target metastasis of malignant tumor in recent years. It has been proven that metastasis of malignant tumor is a nonrandom but highlyorganized and selective process. The aim of this study is by analysing the expression of CC Chemokine Receptor 7 (CCR7) in pulmonary tumor tissue and metastasized lymph nodes in NSCLC, to explore the relationship between the expression of CCR7 in pulmonary tumor tissue and metastasized lymph nodes, and explore the significance.
Methods:
SABC immunohitochemcal staining was used to investigate the expression of CCR7 by rabbit anti-human CCR7 monoclonal antibody, and the specimens were 17 cases of adenocarcinoma, 17 cases of Squamous cell Carcinoma, 12 cases of Adenosquamous Carcinoma, 4 cases of large cell carcinoma and 28 cases of metastasized lymph nodes of lung cancer. Negative control sections use 5 cases of inflammatory pseudotumor and 20 cases of normal lung tissue. Two independent pathologists observed all the specimens in the high power field (*400) of microscope by double blind method.
Results:
1.The expression of CCR7 in pulmonary tumor tissue was remarkably higher than normal lung tissue (P <0.005); 2. The expression of CCR7 between pulmonary tumor tissue and metastasized lymph nodes had no significant differences (P =0.177); 3. The expression of CCR7 had correlation with lymph nodes metastasis, The expression level in lymph nodes metastasis group was significantly higher than that in no lymph nodes metastasis group (P =0.016); 4. Along with the increment that clinical stage, the CCR7 expression had increases the high trend (P =0.003).
Conclusions:
CCR7 is over-expression in carcinoma cell nests and lymph node metastasis . It demonstrates that CCR7 may be related to the development of lymph node metastasis in NSCLC.
Insights
CC Chemokine Receptor 7 (CCR7) is overexpressed in non-small cell lung cancer (NSCLC) tissues and correlates with lymph node metastasis. This suggests CCR7 plays a role in NSCLC
Area of Science:
- Oncology
- Molecular Biology
- Immunology
Context:
- Malignant tumor metastasis is a complex, selective process.
- Non-small cell lung cancer (NSCLC) metastasis is a significant clinical challenge.
- CC Chemokine Receptor 7 (CCR7) is implicated in tumor cell migration.
Purpose:
- To investigate the expression of CCR7 in NSCLC tumor tissues and metastasized lymph nodes.
- To explore the relationship between CCR7 expression and lymph node metastasis in NSCLC.
- To determine the clinical significance of CCR7 in NSCLC progression.
Summary:
- CCR7 expression was significantly higher in NSCLC tumor tissue compared to normal lung tissue.
- CCR7 expression levels in tumor tissue and metastasized lymph nodes showed no significant difference.
- Elevated CCR7 expression correlated with lymph node metastasis and advanced clinical stage in NSCLC.
Impact:
- CCR7 is overexpressed in NSCLC, particularly in carcinoma cell nests and lymph node metastases.
- CCR7 may be a key factor in the development and progression of lymph node metastasis in NSCLC.
- Understanding CCR7's role could lead to targeted therapies for NSCLC metastasis.