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Atmospheric-pressure Molecular Imaging of Biological Tissues and Biofilms by LAESI Mass Spectrometry

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Published on: September 3, 2010

Laserspray ionization using an atmospheric solids analysis probe for sample introduction.

Frank Zydel¹, Sarah Trimpin, Charles N McEwen

¹University of the Sciences in Philadelphia, Philadelphia, Pennsylvania 19194, USA.

Journal of the American Society for Mass Spectrometry

|August 25, 2010

Summary

This summary is machine-generated.

High sensitivity laserspray ionization mass spectrometry (LSI-MS) enables analysis of nonvolatile compounds. This new method uses a simple probe for rapid, high-resolution mass spectra of peptides and proteins.

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Area of Science:

Analytical Chemistry
Mass Spectrometry
Spectroscopy

Background:

The laserspray ionization (LSI) mass spectrometry (MS) method offers high sensitivity for analyzing nonvolatile and high-mass compounds.
Implementation of LSI-MS typically involves specialized equipment for sample introduction.

Purpose of the Study:

To demonstrate the implementation of a simple probe device for LSI-MS.
To showcase rapid analysis of peptides and proteins using LSI-MS with atmospheric pressure (AP) ionization.
To evaluate the performance of LSI-MS with ion mobility spectrometry/MS.

Main Methods:

Utilized a probe device originally designed for atmospheric solids analysis probe (ASAP) method for sample introduction.
Employed laser ablation of a matrix/analyte mixture (2,5-dihydroxybenzoic acid) at atmospheric pressure.
Analyzed peptide and protein samples, including rapid analysis of multiple samples on a single melting point tube.
Acquired mass spectra using high-resolution and ion mobility spectrometry/MS.

Main Results:

Successfully obtained multiply charged ions from nonvolatile peptides and proteins using the LSI-MS method.
Demonstrated the capability of the simple probe device for efficient sample introduction.
Achieved rapid analysis of multiple peptide and protein samples on a single melting point tube.
Obtained high-resolution mass spectra and ion mobility spectrometry/MS data.

Conclusions:

The implemented LSI-MS method with a simple probe device is effective for analyzing peptides and proteins.
This approach facilitates rapid, high-sensitivity analysis of nonvolatile and high-mass compounds.
The integration with ion mobility spectrometry/MS enhances analytical capabilities.