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A novel method for large-scale immuno-SEM using protein G coupled polystyrene beads

In-Hwan Song1, James E Dennis

  • 1Department of Anatomy, College of Medicine, Yeungnam University, 317-1 Daemyungdong, Daegu 705-717, South Korea.

Journal of Electron Microscopy
|August 25, 2010
PubMed

Polystyrene beads with a mean diameter of 0.76 μm were coupled with protein G and then anti-type II collagen IgG or anti-chondroitin-4-sulphate IgG were tagged to protein G. Antibody-tagged beads were applied to articular cartilage and labelled beads were counted in each sample. Antibody-tagged beads labelled significantly higher than IgG isotype control. We propose immuno-SEM using protein G coupled beads as a valuable method for micrometre range observation for specific protein distribution on surfaces of tissues or organs. This will provide information about structure as well as antigenicity on the surface at the same time.

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