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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
Published on: November 15, 2017
Mass spectrometry-based proteomics in cell biology.
Tobias C Walther1, Matthias Mann
1Organelle Architecture and Dynamics and 2 Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Martinsried, Germany. tobias.walther@yale.edu
The Journal of Cell Biology
|August 25, 2010
Summary
Mass spectrometry (MS)-based proteomics enables global analysis of proteins. Advances in sample prep, MS, and computation make this powerful cell biology tool more accessible.
Area of Science:
- Cell Biology
- Biochemistry
- Molecular Biology
Background:
- Global analysis of protein composition, modifications, and dynamics is crucial in cell biology.
- Mass spectrometry (MS)-based proteomics has emerged as a key technology for these analyses.
- High-resolution MS methods are particularly effective for quantitative proteome analysis.
Purpose of the Study:
- To highlight the importance and advancements in MS-based proteomics.
- To emphasize the potential of proteomics as a staple methodology for cell biologists.
Main Methods:
- High-resolution mass spectrometry (MS) for quantitative proteome analysis.
- Advancements in sample preparation techniques.
- Improvements in computational analysis of proteomic data.
Main Results:
- MS-based proteomics is highly successful for quantitative analysis of cellular and organellar proteomes.
- Rapid progress across the entire proteomic workflow is evident.
Conclusions:
- Proteomics technologies are becoming more accessible to a wider scientific community.
- MS-based proteomics is poised to become a standard methodology in cell biology research.
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