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Related Experiment Video

Updated: Jun 9, 2026

Fluorescence Activated Cell Sorting of Plant Protoplasts
13:35

Fluorescence Activated Cell Sorting of Plant Protoplasts

Published on: February 18, 2010

Fluorescence-activated cell sorting in plant developmental biology.

Anjali S Iyer-Pascuzzi1, Philip N Benfey

  • 1Department of Biology and NIH Center for Systems Biology, Duke University, Durham, NC, USA.

Methods in Molecular Biology (Clifton, N.J.)
|August 25, 2010
PubMed
Summary

Fluorescence-activated cell sorting (FACS) isolates plant cells for gene expression analysis. This method aids understanding of organ development under various conditions.

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Area of Science:

  • Plant developmental biology
  • Systems biology
  • Molecular biology

Background:

  • Organ development relies on understanding gene, protein, and metabolite expression within specific cell types and tissues.
  • Fluorescence-activated cell sorting (FACS) is a key technique in plant developmental biology for isolating cells of interest.

Purpose of the Study:

  • To apply FACS for analyzing gene expression profiles in different Arabidopsis root cell types.
  • To investigate gene expression under both standard and stress conditions.

Main Methods:

  • Utilized fluorescence-activated cell sorting (FACS) to isolate specific cell populations from Arabidopsis roots.
  • Analyzed gene expression profiles of sorted cells.
  • Explored downstream proteomic and metabolite analysis applications.

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Cell Specific Analysis of Arabidopsis Leaves Using Fluorescence Activated Cell Sorting
11:25

Cell Specific Analysis of Arabidopsis Leaves Using Fluorescence Activated Cell Sorting

Published on: October 4, 2012

Isolation and Transcriptome Analysis of Plant Cell Types
08:53

Isolation and Transcriptome Analysis of Plant Cell Types

Published on: April 7, 2023

Related Experiment Videos

Last Updated: Jun 9, 2026

Fluorescence Activated Cell Sorting of Plant Protoplasts
13:35

Fluorescence Activated Cell Sorting of Plant Protoplasts

Published on: February 18, 2010

Cell Specific Analysis of Arabidopsis Leaves Using Fluorescence Activated Cell Sorting
11:25

Cell Specific Analysis of Arabidopsis Leaves Using Fluorescence Activated Cell Sorting

Published on: October 4, 2012

Isolation and Transcriptome Analysis of Plant Cell Types
08:53

Isolation and Transcriptome Analysis of Plant Cell Types

Published on: April 7, 2023

Main Results:

  • Established a method to examine gene expression in distinct Arabidopsis root cell types.
  • Demonstrated the utility of FACS for analyzing gene expression under normal and stress conditions.
  • Highlighted the potential for proteomic and metabolite analysis using FACS-isolated cells.

Conclusions:

  • FACS is a powerful tool for dissecting cell-type-specific gene expression in plants.
  • This systems biology approach provides insights into organ development and response to environmental factors.
  • FACS facilitates comprehensive analysis of biological functions at the cellular level.