Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multimodal Ultrasound in Evaluating Radiotherapy Efficacy for Vaginal Squamous Cell Carcinoma: A Case Report.

Current medical imaging·2026
Same author

Machine learning-based prediction of overall survival in non-metastatic renal cell carcinoma after radical nephrectomy.

Scientific reports·2026
Same author

Associationof Static and Dynamic Pupillary Abnormalities with Retinal Microvasculopathy and Neurodegeneration in Diabetics.

Photodiagnosis and photodynamic therapy·2026
Same author

Elevated N/P Ratios Enhance Arsenic Bioaccumulation and Biotransformation in Marine Microalgae.

Environmental science & technology·2026
Same author

Quality and reliability of online health information on benign paroxysmal positional vertigo in TikTok and Bilibili short videos: A cross-sectional content analysis.

Digital health·2026
Same author

The NRT1.1-NLP7 Nexus: An Integrative Signaling Nexus from Nitrate Sensing to Systemic Adaptation and Structure-Guided Engineering.

Plants (Basel, Switzerland)·2026

Related Experiment Video

Updated: Jun 9, 2026

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments
10:59

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Published on: February 7, 2025

Mass spectrometry based glycoproteomics--from a proteomics perspective.

Sheng Pan1, Ru Chen, Ruedi Aebersold

  • 1Department of Pathology, University of Washington, Seattle, WA 98195, USA. span@u.washington.edu

Molecular & Cellular Proteomics : MCP
|August 26, 2010
PubMed
Summary

Glycosylation, a key protein modification, is crucial for health and disease. Glycoproteomics advances biomarker discovery for cancer and other diseases using mass spectrometry.

More Related Videos

A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues
09:16

A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues

Published on: May 4, 2022

Mass Spectrometric Analysis of Glycosphingolipid Antigens
13:09

Mass Spectrometric Analysis of Glycosphingolipid Antigens

Published on: April 16, 2013

Related Experiment Videos

Last Updated: Jun 9, 2026

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments
10:59

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Published on: February 7, 2025

A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues
09:16

A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues

Published on: May 4, 2022

Mass Spectrometric Analysis of Glycosphingolipid Antigens
13:09

Mass Spectrometric Analysis of Glycosphingolipid Antigens

Published on: April 16, 2013

Area of Science:

  • Biochemistry and Molecular Biology
  • Proteomics
  • Glycoscience

Background:

  • Glycosylation is a vital protein post-translational modification impacting numerous physiological processes.
  • Aberrant glycosylation patterns are linked to diseases like cancer, inflammation, and neurodegeneration.
  • Glycoproteins are increasingly recognized as critical biomarkers for disease diagnosis and treatment monitoring.

Purpose of the Study:

  • To provide an overview of recent advancements in mass spectrometry-based glycoproteomic methods.
  • To highlight the role of glycoproteomics in biomarker discovery for clinical applications.
  • To discuss the challenges and potential of identifying and quantifying aberrant glycosylation.

Main Methods:

  • Review of mass spectrometry-based techniques in glycoproteomics.
  • Discussion of methodologies for comprehensive glycoproteome analysis.
  • Exploration of quantitative approaches for detecting glycosylation abnormalities.

Main Results:

  • Recent advances in mass spectrometry have improved the identification and quantification of glycoproteins.
  • Glycoproteomics offers powerful tools for discovering novel biomarkers.
  • Integrated approaches combining various techniques are essential for in-depth analysis.

Conclusions:

  • Mass spectrometry-based glycoproteomics is a rapidly advancing field with significant potential for clinical applications.
  • The discovery of glycoproteins as biomarkers can revolutionize disease diagnosis and prognosis.
  • Further technological development is needed to fully harness the potential of glycoproteomics in medicine.