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Hyperphosphatasemia related to three intestinal alkaline phosphatase isoforms: biochemical study
L Bentouati1, M S Baboli, H Hachem
1Département de Biologie Clinique, Centre Claudius Regaud, Toulouse, France.
Clinica Chimica Acta; International Journal of Clinical Chemistry
|December 13, 1990
Summary
A healthy woman had unexplained high alkaline phosphatase levels. Analysis revealed three intestinal alkaline phosphatase isoforms, suggesting the existence of different allelozymes of this enzyme.
Area of Science:
- Biochemistry
- Enzymology
- Human Physiology
Background:
- Alkaline phosphatase (ALP) is a crucial enzyme involved in various physiological processes.
- Elevated serum ALP levels (hyperphosphatasemia) can indicate underlying health issues, but can also be unexplained.
Observation:
- A 47-year-old healthy female presented with unexplained hyperphosphatasemia.
- Serum analysis using agarose gel electrophoresis, with and without wheat germ lectin, was performed.
Findings:
- The patient's serum displayed three distinct alkaline phosphatase isoforms.
- Biochemical properties (neuraminidase, phenylalanine sensitivity) and thermodenaturation profiles matched those of intestinal tissue.
- Isoelectric point (pI) and pH optima further supported an intestinal origin for these isoforms.
Implications:
- The findings suggest the presence of multiple allelozymes of intestinal alkaline phosphatase in humans.
- This case highlights the importance of characterizing ALP isoforms in unexplained hyperphosphatasemia.
- Further research into ALP allelozymes could refine diagnostic approaches for liver and bone diseases.