Related Experiment Video
Updated: Aug 9, 2026

Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting
Published on: December 12, 2011
Analytical methodology for immunoassays and DNA hybridization assays--current status and selected systems--critical
1Department of Clinical Biochemistry, Toronto Western Hospital, Ontario, Canada.
Abstract:
Immunoassay is an established technique which has contributed enormously to biomedical analysis. DNA hybridization (DNA probing) methodology is emerging as the most promising new discipline of laboratory medicine with potential applications in areas such as genetics, pathology, microbiology and oncology. In this review, immunoassay and DNA probing methodologies are considered together because of their many similarities in assay design and labeling systems. Selected labeling systems are described in this paper in order to stress strategies, general principles and future trends. Special attention has been given to systems that introduce linear or exponential amplification. In the author's view, such systems will dominate in future applications. It is anticipated that during the next decade, immunoassay and DNA probing assays will be carried out on completely automated systems.
Related Concept Videos
DNA Microarrays
Immunoprecipitation
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...
Enzyme-Linked Immunosorbent Assay
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.

