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A simple procedure for determination of the dead time of a stopped-flow instrument
K Matsumura1, Y Enoki, H Kohzuki
1Second Department of Physiology, Nara Medical University, Kashihara, Japan.
The Japanese Journal of Physiology
|January 1, 1990
Abstract:
The reducing reaction of 2,6-dichlorophenolindophenol by L-ascorbic acid was used to determine the dead time of a stopped-flow instrument. Because this reaction is irreversible, the dead time could be determined by a simple graphical analysis. The dead time values determined by the present method were comparable to those by other methods previously reported.